176,041 results

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Tet-inducible mCherry reporter

Plasmid

#64128

Purpose

Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

mCherry

Expression

Mammalian

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSBbi-GN

Plasmid

#60517

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

Depositor

Eric Kowarz

Publication

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pGL3-Basic-8x-gRNA-eGFP

Plasmid

#60718

Purpose

eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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single polypeptide FPX biosensor for calcium ion

Plasmid

#60887

Purpose

FPX biosensor

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for calcium ion

Tags

HindIII site

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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tol2-mpeg1-mcherry

Plasmid

#58935

Purpose

labels macrophages with mcherry

Depositor

Anna Huttenlocher

Insert

mpeg1-mcherry

Promoter

mpeg1

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Publication

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Publication

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

Plasmid

#52421

Purpose

Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

Depositor

Bertrand Collet

Insert

Fusion NLS-EGFP-P2A-Cherry-CAAX

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMDC32B-AtMIR390a-B/c

Plasmid

#51776

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Publication

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Plant expression

Mutation

A. thaliana MIR390a precursor sequence including …

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Expression

Mammalian

Mutation

S257G in Rluc8

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA-HA-ER Y537S

Plasmid

#49499

Purpose

Expresses HA-tagged ER Y537S mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Publication

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Tyrosine 537 to Serine

Promoter

CMV

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Publication

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Publication

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Publication

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Insert

ERK2 (MAPK1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
HBM-Luc

Plasmid

#35155

Depositor

Linda Penn

Publication

Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

Insert

Myc promoter (MYC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains 2.5 kb of the c-myc minimal promoter

Promoter

c-myc

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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2.2

Plasmid

#34885

Depositor

Irvin Chen

Publication

Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

Insert

Sindbis virus envelope protein

Use

Lentiviral

Expression

Mammalian

Mutation

SGN UTR in E1 domain

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Publication

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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