4,177 results

1. lentiCRISPR v2

   Plasmid

   #52961

   Purpose

   Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-Chk1-Flag

   Plasmid

   #22894

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Chk1 (CHEK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-VSV-G

   Plasmid

   #8454

   Purpose

   Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV(CAT)T7-SB100

   Plasmid

   #34879

   Depositor

   Zsuzsanna Izsvak

   Article

   Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

   Insert

   SB100X transposase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 35S:RUBY

   Plasmid

   #160908

   Purpose

   RUBY under the control of CaMV 35S promoter/marker for transformation

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and CaMV 35S promoter
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUMVC

   Plasmid

   #8449

   Purpose

   Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 4, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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