2,279 results

1. pCI-EGFP-NR2b wt

   Plasmid

   #45447

   Depositor

   Andres Barria, Robert Malinow

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR2B (Grin2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-EGFP-NR2a wt

   Plasmid

   #45445

   Depositor

   Andres Barria, Robert Malinow

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR2A (Grin2a Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Mammalian expression: Z-lock cofilin dark state mutant

   Plasmid

   #141136

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs GFP-LC3-RFP

   Plasmid

   #117413

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-C1-PKCgamma-C1A

   Plasmid

   #21205

   Depositor

   Tobias Meyer

   Article

   Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

   Insert

   PKCgamma-C1A (Prkcg Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PKCgamma-C1A domain. (Lab plasmid WC 0018)

   Promoter

   Available Since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mEGFP(A206K)-paCaMKII

   Plasmid

   #165438

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mEGFP(A206K)-paCaMKII(K42M)

   Plasmid

   #165440

   Purpose

   Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(K42M)
   

   Use

   Tags

   mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   K42M: Kinase dead mutation

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-C1(2)delta

   Plasmid

   #21216

   Depositor

   Tobias Meyer

   Article

   Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

   Insert

   GFP-N2-PKCdelta-C1 (Prkcd Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C1(2) domains of PKC delta (rat) (Lab plasmid WC…

   Promoter

   Available Since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. HMA-APOBEC1-Nb

   Plasmid

   #226012

   Purpose

   For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

   Depositor

   Eugene Yeo

   Article

   Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Tags

   His-MBP and antiRabbit-nanobody

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK7-HA-S6K1-F5A-E389-R3A

    Plasmid

    #8991

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

    Promoter

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF Synaptophysin-mRuby

    Plasmid

    #188980

    Purpose

    Synaptic buton marker

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Rat, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. VGluT1-SNAPtag

    Plasmid

    #107371

    Purpose

    Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    Vesicular Glutamate transporter 1 (Slc17a7 Rat)
    

    Use

    Tags

    SNAPtag, 104 aa before C term

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-RUSH-LAMP1-V5-APEX2

    Plasmid

    #228524

    Purpose

    Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP), Streptavidin-…

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Myc-GRM1-IRES-CyRFP1

    Plasmid

    #195355

    Purpose

    mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    GRM1-IRES-CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PKC zeta DN

    Plasmid

    #21249

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281M

    Promoter

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

18. rGluA2 (px330:2-guideCas9)

    Plasmid

    #169439

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHFS:SEP-rGluA2 TKIT donor

    Plasmid

    #169440

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA2 in rat

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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