2,306 results
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iRFP-PH-PLCdelta1
Plasmid#66841Purposeexpression of near-infrared PI(4,5)P2 biosensorDepositorInsertPH-PLCdelta1
TagsiRFPExpressionMammalianPromoterCMVAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/F-tractin=mStayGold
Plasmid#212019PurposeFor filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertF-tractin
TagsmStayGoldExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-OMP25
Plasmid#141150PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorInsertOMP25 (Synj2bp Rat)
TagsAcGFPExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6f
Plasmid#40755PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6f
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenSyn2-EGFP-OMP25C
Plasmid#71427PurposeLentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.DepositorInsertOMP25 (172-‐206) (Synj2bp Rat)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 172-206 onlyPromotersynapsinAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE2
Plasmid#73020PurposeExpresses BE2 in mammalian cellsDepositorInsertBE2
ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2B
Plasmid#17925DepositorAvailable SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
TagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)Available SinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2A
Plasmid#17924DepositorAvailable SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLIK S6K (E389-deltaCT) neo
Plasmid#58516PurposeLentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseLentiviralTagsHAExpressionMammalianMutationT389E, stop codon at amino acid 402Available SinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GCaMP5G
Plasmid#31788Purposea single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.DepositorInsertGCaMP5G
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDS170-enNTS1
Plasmid#199152PurposeJZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)DepositorInsertenNTS1 (Ntsr1 Rat)
Tags10xHis tag, Avi tag, MBP, and muGFPExpressionBacterialMutationV208M, C273S, C391S and C393S (over NTS1-B5)PromoterLac/T5Available SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold2t-Lysosome-N-20
Plasmid#231778PurposeVisualization of lysosome in mammalian cells using mGold2tDepositorAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-LCa
Plasmid#53972PurposeExpresses the light chain of clathrinDepositorInsertclathrin light chain
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-ERK2-MEK1_R4F_coexpression
Plasmid#39212DepositorTagsHis6 and RBSExpressionBacterialMutationMEK1(R4F) = S218E, S222D and deletion of AA32-51PromoterT7 and T7 via RBSAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR1a
Plasmid#17926DepositorInsertglutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
TagseGFPExpressionMammalianAvailable SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only
