2,362 results

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pCI-EGFP-NR2b wt

Plasmid

#45447

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2B (Grin2b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR2a wt

Plasmid

#45445

Depositor

Andres Barria, Robert Malinow

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR2A (Grin2a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFR3.v857.SGZ

Plasmid

#178330

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV hSyn iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6s

Plasmid

#40753

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Insert

GCaMP6s

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SEP-Glur2 (MCA10)

Plasmid

#64941

Purpose

mammalian expression of rat GluR2

Depositor

Jeremy Henley

Article

Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

Insert

GluR2 (Gria2 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 mCherry

Plasmid

#105010

Purpose

Excitatory presynaptic marker

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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wt HA-dynamin 2 pcDNA3.1

Plasmid

#34684

Depositor

Sandra Schmid

Insert

dynamin 2ba

Tags

HA (variant)

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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Syn-ATP

Plasmid

#51819

Purpose

Optical reporter of presynaptic ATP

Depositor

Timothy Ryan

Article

Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

Insert

Chimeric Synaptophysin-mCherry-Luciferase

Expression

Mammalian

Mutation

Within WT luciferase gene, changed Threonine 214 …

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-LC3-RFP-LC3ΔG

Plasmid

#168997

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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