2,301 results

1. pGP-CMV-GCaMP6f

   Plasmid

   #40755

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6f
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-NR2B

   Plasmid

   #17925

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   N-methyl D-aspartate receptor 2B (Grin2b Rat)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs GFP-LC3-RFP

   Plasmid

   #117413

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmcherry-LCa

   Plasmid

   #53972

   Purpose

   Expresses the light chain of clathrin

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   clathrin light chain
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

5. VAChT-pHuji

   Plasmid

   #105319

   Purpose

   VAChT tagged with pH-sensitive red fluorescent protein pHuji

   Depositor

   Justin Taraska

   Article

   Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

   Insert

   VAChT (Slc18a3 Rat)
   

   Tags

   pHuji

   Expression

   Mammalian

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-NR2A

   Plasmid

   #17924

   Depositor

   Stefano Vicini

   Article

   Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

   Insert

   NMDAR2A (Grin2a Rat)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Myr.PKCzeta.FLAG

   Plasmid

   #10802

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Tags

   FLAG and Myr

   Expression

   Mammalian

   Available Since

   July 26, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pORANGE Cacnb3-GFP KI

   Plasmid

   #139662

   Purpose

   Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-NR1a

    Plasmid

    #17926

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEAK10-His-Myc-Kal12

    Plasmid

    #25442

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal12
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pEAK10-His-Myc-Kal9

    Plasmid

    #25441

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal9
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pEAK10-His-Myc-Kal5

    Plasmid

    #25440

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal5
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pEAK10-His-Myc-Kal7

    Plasmid

    #25454

    Depositor

    Betty Eipper

    Article

    Penzes et al J Biol Chem. 2000 Mar 3. 275(9):6395-403

    Insert

    Kal7
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-GFP-Rim2

    Plasmid

    #170869

    Purpose

    Mammalian expression of His-GFP-Rim2.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD454-SR rat Alpha-synuclein

    Plasmid

    #89074

    Purpose

    bacterial expression of rat Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Rat)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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