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Myo 9a-GFP

Plasmid

#134959

Purpose

Expression of rat Myo 9a (Myr7) in mammalian cells.

Depositor

Martin Bähler

Article

Chieregatti et al J Cell Sci. 1998 Dec 18;111 ( Pt 24):359

Insert

Myo 9a (rat) with 5' UTR (Myo9a Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

Plasmid

#215014

Purpose

Express an autophagy (LC3) reporter in mammalian cells (untested)

Depositor

Wade Harper

Insert

MAP1LC3B (Map1lc3b Rat)

Use

Lentiviral

Tags

HaloTag(7)-mGFP

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-GCaMP6s

Plasmid

#105714

Purpose

Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

Depositor

Rylan Larsen

Has Service

AAV8

Insert

GCaMP6s

Use

AAV; Flp/frt

Expression

Mammalian

Promoter

Ef1a, Human elongation factor-1 alpha

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTrcHisA-rat-PTP1B

Plasmid

#35987

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

His and Xpress Epitope Tag

Expression

Bacterial

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mRuby3

Plasmid

#155221

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b

Tags

mRuby3

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-trkB.DN-mCherry

Plasmid

#121502

Purpose

Expresses trkB.T1 fused with mCherry in a cre dependent manner.

Depositor

Marie Carlén

Article

Guyon et al J Neurosci. 2021 Feb 15. pii: JNEUROSCI.

Insert

trkB.T1 (Ntrk2 Rat)

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TrkB

Plasmid

#32500

Depositor

Rosalind Segal

Insert

TRKB (Ntrk2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkB

Plasmid

#39978

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB (Ntrk2 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

Plasmid

#104492

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

Insert

jGCaMP7f

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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GLUT1-GFP

Plasmid

#89440

Purpose

GLUT1 construct

Depositor

Jeffrey Rathmell

Article

Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

Insert

GLUT1 (Slc2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc rat SAPAP1

Plasmid

#40215

Depositor

Yutaka Hata, Yoshimi Takai

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP1 (Dlgap1 Rat)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCMV Myc rat SAPAP4

Plasmid

#40218

Depositor

Yutaka Hata, Yoshimi Takai

Article

Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

Insert

SAPAP4 (Dlgap4 Rat)

Tags

myc

Expression

Mammalian

Mutation

N834D

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
HS-aPKC-CAAX-GFP

Plasmid

#105946

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC (Prkcz Rat)

Tags

CAAX and GFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAE-MIBP1

Plasmid

#51873

Purpose

Expresses full length rat MIBP1 (cloned in pCAGGS)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Fukuda et al J Biochem. 2002 Mar;131(3):349-57.

Insert

MIBP1 (Hivep2 Rat)

Tags

none

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
GFP-GLT1a

Plasmid

#162515

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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