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pLenti sfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164478

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-IRES2-p75NTR-GFP

Plasmid

#182947

Purpose

To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

Depositor

Pantelis Tsoulfas

Insert

p75NTR-mGFP

Tags

HA tag and mGFP

Expression

Mammalian

Mutation

The leader sequence is from preprotrypin

Promoter

CAG

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkA

Plasmid

#39977

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkA (Ntrk1 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

endogenous signal sequence was replaced with BM40…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rBcan

Plasmid

#18958

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican (Bcan Rat)

Expression

Mammalian

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pB-Neo-rTOM20-GFP

Plasmid

#210331

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mNeonGreen

Plasmid

#196864

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mNeonGreen (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 F-tractin-EGFP

Plasmid

#58473

Purpose

Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

F-tractin (Itpka Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-SEP-GluA1

Plasmid

#227235

Purpose

Large cargo capacity AAV that expresses SEP tagged AMPA receptor subunit GluA1 in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

SEP-GluA1 (Gria1 Rat)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSEP-LAMP1-RFP

Plasmid

#167970

Purpose

Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

Depositor

Juan Bonifacino

Article

Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

LAMP1 (Lamp1 Rat)

Tags

SEP and mRFP

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Kif5A-GFP-CIBN

Plasmid

#102252

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Kif5A-CIBN

Plasmid

#102251

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM60-eIF4E

Plasmid

#38239

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

eIF4E (Eif4e Rat)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SEP-Glur2 (MCA10)

Plasmid

#64941

Purpose

mammalian expression of rat GluR2

Depositor

Jeremy Henley

Article

Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

Insert

GluR2 (Gria2 Rat)

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Promoter

UbC

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

Plasmid

#71878

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC-delta (Otc Rat)

Mutation

Deleted amino acid 30-114 from full length OTC

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP6fu

Plasmid

#86235

Purpose

Express fluorescent calcium indicator GCaMP6fu in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

Insert

GCaMP6fu

Expression

Mammalian

Mutation

W43Y D395A

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/F-tractin=mStayGold

Plasmid

#212019

Purpose

For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

F-tractin

Tags

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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