We narrowed to 59,899 results for: %s

1. Crimson/P2A-ICP8 (pSLIK1)

   Plasmid

   #113856

   Purpose

   For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   ICP8
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Crimson/P2A-Control (pSLIK3)

   Plasmid

   #113858

   Purpose

   An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   E2-Crimson
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI5-CasRx-HA (pKW88)

   Plasmid

   #229046

   Purpose

   Mammalian expression of CasRx-HA

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Insert

   CasRx (RfxCas13d)
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG5-BRLF1

   Plasmid

   #72635

   Purpose

   Mammalian expression vector for Epstein-Barr virus Rta immediate-early replication and transcription activator

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BRLF1
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAF123

   Plasmid

   #135921

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Available Since

   Jan. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETB-roGFP1-R12

   Plasmid

   #83311

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

   Insert

   roGFP1-R12
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

   Promoter

   T7

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pSG5-BMLF1

   Plasmid

   #72636

   Purpose

   Mammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulator

   Depositor

   S. Diane Hayward

   Article

   Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

   Insert

   BMLF1/BSLF2
   

   Expression

   Mammalian

   Mutation

   Q157H

   Available Since

   Feb. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pEGFP-N1-ro1GFP

   Plasmid

   #82369

   Purpose

   Expression of redox sensitive GFP in mammalian cells

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro1GFP
   

   Expression

   Mammalian

   Mutation

   C48S/F64L/T65S/S147C/Q204C

   Promoter

   CMV

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAF92

    Plasmid

    #135929

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    mCherry fused to TNFSF9
    

    Use

    Transposon

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDual-eGFP(Y203)

    Plasmid

    #63559

    Purpose

    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP(Y203)
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Changed threonine at position 203 with respect to…

    Promoter

    CMV-EF1α hybrid (CEF)

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

    Plasmid

    #217512

    Purpose

    KD Recombinase dependent conditional smGdP-10XOllas cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
    

    Tags

    smGdP-10XOllas

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nsp3 Mac1 - P43 (Oxford)

    Plasmid

    #169210

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pEGFP-N1-mt-ro1GFP

    Plasmid

    #82407

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro1GFP
    

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/T65S/S147C/Q204C

    Promoter

    CMV

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mAMCase_CatD_6xHis_pTwistCMV

    Plasmid

    #200228

    Purpose

    Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pTwist CMV vector

    Depositor

    James S Fraser

    Article

    Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

    Insert

    Acidic Mammalian Chitinase (Chia1 Mouse)
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S354F

    Promoter

    CMV

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-PspCas13b-non-targeting (pJK5)

    Plasmid

    #229052

    Purpose

    Cloning backbone for PspCas13b crRNA (U6 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

    Plasmid

    #217511

    Purpose

    KD Recombinase dependent conditional smGdP-10XHA cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
    

    Tags

    smGdP-10XHA

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmU1-CasRx-non-targeting (pKW83)

    Plasmid

    #229051

    Purpose

    Cloning backbone for CasRx crRNA (Mouse U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ICP8-P2A/Crimson (pSLIK4)

    Plasmid

    #113859

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSETB-de4GFP

    Plasmid

    #85472

    Purpose

    Inducible expression of dual emitting pH sensitive GFP in bacterial cells

    Depositor

    S. James Remington

    Article

    Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

    Insert

    de4GFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S65T/C48S/H148C/T203C

    Promoter

    T7

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge