We narrowed to 1,991 results for: CCS
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Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianPromoterhuman PGK1Available SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only
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Cre-IRES-PuroR
Plasmid#30205PurposeLentiviral coexpression of Cre and puromycin from the human EF1a promoterDepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseCre/Lox and LentiviralExpressionMammalianPromoterEF1alphaAvailable SinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_No_FLAG_AAVS1_T2
Plasmid#79888PurposeExpresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.DepositorTypeEmpty backboneUseCRISPRTagsUntagged eSpCas9(1.1)ExpressionMammalianPromoterCBhAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnExpressionMammalianPromoterTet-On 3G BidirectionalAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cas9)
Plasmid#174864PurposeExpresses human Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by HsPeg10 UTRs
TagsHA-SV40 NLS and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-PE7 for IVT
Plasmid#214813PurposeTemplate for in vitro transcription of PE7DepositorInsertPE7
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSPromoterT7 (inactivated)Available SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7
Plasmid#214812PurposeMammalian expression of SpCas9 PE7 prime editorDepositorInsertPE7
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianPromoterCMVAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.PAC
Plasmid#57828PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai-IRES-mCherry
Plasmid#72894PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherryDepositorInsertdmBACCS2-IRES-dOrai-IRES-mCherry
TagsIRES-mCherryExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChR2(H134R)-eYFP
Plasmid#127090PurposeCre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activationDepositorHas ServiceAAV PHP.eBInsertChannelrhodopsin-2
UseAAVTagseYFP (C terminal on insert)ExpressionMammalianMutationHumanized ChR2 gene with histidine 134 changed to…PromoterCAGAvailable SinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304 Flag-mCherry-eEF1A1_IRES-GFP
Plasmid#198383PurposeeEF1A1 fluorescent reporterDepositorAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dyn2-pmCherryN1
Plasmid#27689DepositorAvailable SinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ERBB3
Plasmid#23874DepositorInsertERBB3 (ERBB3 Human)
UseGateway CloningAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-mKate2 caspase-3 biosensor
Plasmid#37132DepositorInsertLSSmOrange-DEVD-mKate2
ExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLSSmOrange-N1
Plasmid#37130DepositorInsertLSSmOrange
ExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable SinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIPK1 gamma 90
Plasmid#22299DepositorInserthuman phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
TagsGFPExpressionBacterial and MammalianAvailable SinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.BSD
Plasmid#57821PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.PAC
Plasmid#58329PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only