We narrowed to 12,214 results for: LEA

Showing: 41 - 60 of 12214 results

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Publication

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

74 Articles

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pY010 (pcDNA3.1-hAsCpf1)

Plasmid

#69982

Purpose

Expresses humanized AsCpf1

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hAsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

61 Articles

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pCMV-BE3

Plasmid

#73021

Purpose

Expresses BE3 in mammalian cells

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE3

Expression

Mammalian

Promoter

CMV

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

78 Articles

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pcDNA3.1-p300

Plasmid

#23252

Depositor

Warner Greene

Publication

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p300 (EP300 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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HA-p62

Plasmid

#28027

Purpose

Mammalian expression of HA tagged human p62

Depositor

Qing Zhong

Publication

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

p62 (SQSTM1 Human)

Tags

HA

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

66 Articles

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Publication

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

49 Articles

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pCMV-ABE7.10

Plasmid

#102919

Purpose

expresses ABE7.10 in mammalian cells

Depositor

David Liu

Publication

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE7.10

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

40 Articles

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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GFP-RelA

Plasmid

#23255

Depositor

Warner Greene

Publication

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p65 (RELA Human)

Tags

EGFP

Expression

Mammalian

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Publication

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

193 Articles

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pCAG-EGxxFP

Plasmid

#50716

Purpose

5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Publication

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

split EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

64 Articles

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pCS2-nCas9n

Plasmid

#47929

Purpose

expression of an optimized Cas9 for genome editing in zebrafish

Depositor

Wenbiao Chen

Publication

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nls-zcas9-nls

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

104 Articles

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GFP-p53

Plasmid

#12091

Depositor

Tyler Jacks

Publication

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p53 (TP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Available since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Publication

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

51 Articles

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nls-NgAgo-GK

Plasmid

#78253

Purpose

Mammalian expression of NgAgo (Argonaute from N. gregoryi).

Depositor

Chunyu Han

Publication

Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

NgAgo

Tags

a nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Publication

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Tags

NT* solubility tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pcDNA3-EGFP-C4-Nrf2

Plasmid

#21549

Depositor

Yue Xiong

Publication

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)

Tags

EGFP and Myc

Expression

Mammalian

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pEGFP-C1-ER alpha

Plasmid

#28230

Purpose

Mammalian expression of Estrogen Receptor alpha fused to EGFP

Depositor

Michael Mancini

Publication

Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Estrogen Receptor alpha (ESR1 Human)

Tags

EGFP

Expression

Mammalian

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

29 Articles

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