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pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC475 - pAAV c-fos iRFP

Plasmid

#47906

Purpose

An AAV vector that expresses iRFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

c-fos

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pOTTC364 - pAAV CaMKIIa iRFP

Plasmid

#47903

Purpose

An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pOttc1501 - pscAAV CMV-IE hCDNF

Plasmid

#188538

Purpose

An AAV packaging vector that expresses hCDNF under control of the CMV-IE promoter.

Depositor

Brandon Harvey

Insert

hCDNF (CDNF Human)

Use

AAV

Tags

Expression

Mutation

Promoter

CMV-IE

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1596 - pAAV SYN1 HA-hM3D(Gq)

Plasmid

#121539

Purpose

An adeno-associated viral vector expressing HA-tagged stimulatory DREADD (hM3D(Gq)) from a synapsin promoter

Depositor

Christopher Richie

Article

Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

Has Service

AAV2 and AAV5

Insert

HA-tagged hM3D(Gq) (CHRM3 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

SYN1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC414 - pAAV EF1a hPREP

Plasmid

#59967

Purpose

An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA human, Human)

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC841 - pAAV minP secNLuc

Plasmid

#82498

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

c-Fos minimal promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC592 - pCMV Mito-iRFP

Plasmid

#59135

Purpose

A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Mitochondria-localized Infrared Fluorescent Protein

Use

Tags

Mitochondria-targeting sequence

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC591 - pCMV Mem-iRFP

Plasmid

#59134

Purpose

A plasmid that express membrane-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Membrane-localized Infrared Fluorescent Protein

Use

Tags

Palmitylation signal

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pOttc1495 - pAAV CaMKII SERCaMP_ASARTDL

Plasmid

#192602

Purpose

An AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.

Depositor

Brandon Harvey

Insert

SERCaMP

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRa

Plasmid

#134990

Purpose

dCas9-mediated activation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Top-T~Bottom-T

Plasmid

#87315

Purpose

Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system (promoter control)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pOttc556 - pAAV EF1a iCre

Plasmid

#210416

Purpose

AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

Depositor

Brandon Harvey, Christopher Richie

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1751 - pcDNA3.1 CMV-IE V5-ER-LBD

Plasmid

#201818

Purpose

A plasmid expressing the ligand-binding domain of estrogen receptor with a V5 epitope tag from a CMV promoter

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

V5-ER-LBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRi

Plasmid

#134989

Purpose

dCas9-mediated inactivation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC374 - pAAV EF1a DIO iRFP

Plasmid

#47626

Purpose

An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pOTTC763 - pX458 with rat Rosa26 gRNA A

Plasmid

#113161

Purpose

A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat Rosa26

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

Plasmid

#113163

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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