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Showing: 1451 - 1500 of 18908 results

  3. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid
    #141237
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    GCaMP6f
    Use
    AAV and Cre/Lox
    Tags
    6XHIS and Xpress
    Expression
    Mutation
    Promoter
    Human elongation factor-1 alpha (EF-1 alpha)
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    141237-plasmid-map-sequence-id-431695

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  4. pHR-LS

    Plasmid
    #205438
    Purpose
    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes
    Depositor
    Masamichi Kamihira
    Article
    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478
    Inserts
    Gaussia luciferase gene
    spectinomycin resistance gene
    Use
    Cre/Lox and Luciferase; Chlamydomonas expression
    Tags
    Expression
    Mutation
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205438-plasmid-map-sequence-id-405460

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  5. pGem-LP1-loxP-bar-lox2272-71

    Plasmid
    #168785
    Purpose
    Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168785-plasmid-map-sequence-id-350000

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  6. pGem-LP2-loxP-bar-lox2272-71

    Plasmid
    #168786
    Purpose
    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102
    Inserts
    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    168786-plasmid-map-sequence-id-353782

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  8. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177672-plasmid-map-sequence-id-348533

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  10. R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180152
    Purpose
    tdTomato cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180152-plasmid-map-sequence-id-355317

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  11. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

    Plasmid
    #178049
    Purpose
    CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178049-plasmid-map-sequence-id-352988

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  12. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

    Plasmid
    #178048
    Purpose
    CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178048-plasmid-map-sequence-id-352985

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  13. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

    Plasmid
    #178047
    Purpose
    CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Lrig1 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178047-plasmid-map-sequence-id-352935

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  15. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180156
    Purpose
    tdTomato cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180156-plasmid-map-sequence-id-360161

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  23. pCALNL-Rheb(S16H)

    Plasmid
    #194204
    Purpose
    Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.
    Depositor
    Angelique Bordey
    Article
    Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113
    Insert
    Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    S16H
    Promoter
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194204-plasmid-map-sequence-id-382462

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  31. AAV pCAG-FLEX-mScarlet-WPRE

    Plasmid
    #99280
    Purpose
    Can be used to generate AAV virus that will express mScarlet in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mScarlet
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99280-plasmid-map-sequence-id-181816

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  33. pAAV-nEF-Con/Foff DREADD Gi-mCherry

    Plasmid
    #177673
    Purpose
    Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177673-plasmid-map-sequence-id-348532

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  34. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107737
    Purpose
    In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107737-plasmid-map-sequence-id-207281

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  35. p226-AAV-dlx-dio-mScarlet-T2A-SYPEGFP

    Plasmid
    #185696
    Purpose
    AAV vector expressing cre-dependent mem-mScarlet, T2A, Synaptophysin-EGFP driven by Dlx promoter
    Depositor
    Lynette Lim
    Insert
    mScarlet, T2A, Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    mScarlet palmitoylation, Synaptophysin fused to E…
    Expression
    Mammalian
    Mutation
    Promoter
    mDlx
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185696-plasmid-map-sequence-id-366332

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  38. pAAV-hSyn-Flex-Axon-EGFP

    Plasmid
    #135429
    Purpose
    Can be used to drive axon-enriched expression of EGFP in the presence of Cre recombinase.
    Depositor
    Rylan Larsen
    Has Service
    AAV1
    Insert
    axon-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GAP43 palmitoylation domain
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Feb. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135429-plasmid-map-sequence-id-271167

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  40. AAV pCAG-FLEX-mRuby3-WPRE

    Plasmid
    #99279
    Purpose
    Can be used to generate AAV virus that will express mRuby3 in the presence of Cre
    Depositor
    Rylan Larsen
    Insert
    mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99279-plasmid-map-sequence-id-181812

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  41. PB CAG-F-intron-L-F-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #178433
    Purpose
    CAG tdGFP FLP/FRT cre/lox "NOT" reporter piggyBac transposon (bpA SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox; Flp/frt, piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178433-plasmid-map-sequence-id-350247

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  42. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180151
    Purpose
    td-sfGFP cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180151-plasmid-map-sequence-id-355316

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  43. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107736
    Purpose
    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107736-plasmid-map-sequence-id-207278

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  44. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180155
    Purpose
    td-sfGFP cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180155-plasmid-map-sequence-id-355320

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  45. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  46. pSicoR-Ef1a-mCh

    Plasmid
    #31847
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.
    Depositor
    Bruce Conklin
    Article
    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA; Ef1alpha for mCherry
    Available since
    Jan. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    31847-plasmid-map-sequence-id-35883

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  48. LV-indLS2

    Plasmid
    #123068
    Purpose
    Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.
    Depositor
    Kevin Brindle
    Article
    Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1
    Inserts
    CreERT2 with intron
    Firefly luciferase
    mStrawberry
    Use
    Lentiviral and Luciferase; Fluorescence
    Tags
    Expression
    Mammalian
    Mutation
    synthetic intron added as indicated
    Promoter
    CAGGS (after Cre mediated inversion) and CAGGS (f…
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123068-plasmid-map-sequence-id-240523

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  49. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid
    #189630
    Purpose
    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.
    Depositor
    Jerzy Szablowski
    Article
    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Inserts
    Gaussia luciferase fused to Fc
    IRES-EGFP
    Use
    AAV, Cre/Lox, and Luciferase
    Tags
    Gluc and IgG1 Fc
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and hSyn
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    189630-plasmid-map-sequence-id-376528

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Showing: 1451 - 1500 of 18908 results