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Showing: 581 - 600 of 13526 results

  1. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  2. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  4. pLT 653

    Plasmid
    #59996
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    partial genomic
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59996-plasmid-map-sequence-id-110769

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  5. pLT 651

    Plasmid
    #59998
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    klp-16 (klp-16 Nematode)
    him-8 (him-8 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    partial genomic
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59998-plasmid-map-sequence-id-110771

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  6. MSCV-opEBNA2-IRES-mCherry

    Plasmid
    #220355
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220355-plasmid-map-sequence-id-437048

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  8. pLT 652

    Plasmid
    #59997
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    him-8 (him-8 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    partial genomic
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59997-plasmid-map-sequence-id-110770

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  10. MSCV-opEBNA2-IRES-GFP

    Plasmid
    #220354
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    3 x HA tag
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220354-plasmid-map-sequence-id-437068

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  11. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Article
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    102562-plasmid-map-sequence-id-203161

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  13. pcDNA3.1(-)-V5.mER.hOr47a

    Plasmid
    #126473
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER) and V5 tag
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126473-plasmid-map-sequence-id-246528

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  14. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid
    #185910
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185910-plasmid-map-sequence-id-363739

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  15. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid
    #185915
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185915-plasmid-map-sequence-id-367353

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  16. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid
    #126472
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126472-plasmid-map-sequence-id-246570

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  18. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185911-plasmid-map-sequence-id-367364

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  19. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

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  20. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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Showing: 581 - 600 of 13526 results