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GFP-Epac2

Plasmid

#118314

Purpose

Expression of mouse Epac2

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

Insert

Epac2 (Rapgef4 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_UAS-tTS-P2A-GFP_PGK-BFP

Plasmid

#133808

Purpose

inducible expression of tTS and GFP with BFP marker in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

tTS-P2A-EGFP-PGK-BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pG3

Plasmid

#162604

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKM495 (Flag-Das+4) Zeo

Plasmid

#109284

Purpose

ORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-DAS+4

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed trigger

Plasmid

#107357

Purpose

Trigger sequence for pNP1 eforRed toehold switch

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 1

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0826-mCherryBb

Plasmid

#118242

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0826 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0826 promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pJB06

Plasmid

#190480

Purpose

Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Cas9
XylR

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Promoter

xylR-driven promoter

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJB07

Plasmid

#190481

Purpose

Targeting plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Upstream & Downstream pyrE deletion region
gRNA

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pABY-c15

Plasmid

#201768

Purpose

Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pC0091

Plasmid

#181955

Purpose

Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xFOP

Expression

Mammalian

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Promoter

PtetA

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLG002 (BREX type I)

Plasmid

#157880

Purpose

Expresses the BREX type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

BREX type I

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptet1

Plasmid

#218367

Purpose

Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-L

Plasmid

#234657

Purpose

Expresses L gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV L gene (L Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-P

Plasmid

#234656

Purpose

Expresses P gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV P gene (Nvgp2 Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-N

Plasmid

#234655

Purpose

Expresses N gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV N gene (N Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1180-NiV L(2k)-mCherry/eGFP

Plasmid

#234654

Purpose

Minigenome plasmid of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Inserts

part L gene
mCherry
eGFP

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLG003 (Druantia type I)

Plasmid

#157881

Purpose

Expresses the Druantia type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Druantia type I

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1

Plasmid

#183361

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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