9,155 results

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCN5 (KAT2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Publication

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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single polypeptide FPX biosensor for calcium ion

Plasmid

#60887

Purpose

FPX biosensor

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

single polypeptide FPX biosensor for calcium ion

Tags

HindIII site

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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tol2-mpeg1-mcherry

Plasmid

#58935

Purpose

labels macrophages with mcherry

Depositor

Anna Huttenlocher

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mpeg1-mcherry

Promoter

mpeg1

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Publication

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

Plasmid

#52421

Purpose

Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

Depositor

Bertrand Collet

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fusion NLS-EGFP-P2A-Cherry-CAAX

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nano-lantern

Expression

Mammalian

Mutation

S257G in Rluc8

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA-HA-ER Y537S

Plasmid

#49499

Purpose

Expresses HA-tagged ER Y537S mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Publication

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Tyrosine 537 to Serine

Promoter

CMV

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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M-ST1n-VP64

Plasmid

#48675

Purpose

Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null (D9A D598A H599A N622A)

Promoter

CMV

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Flag Depdc5 pRK5

Plasmid

#46340

Depositor

David Sabatini

Publication

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Depdc5 (DEPDC5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pNLS-iRFP713

Plasmid

#45468

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP713

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Publication

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-ERK2

Plasmid

#37145

Depositor

Rony Seger

Publication

Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERK2 (MAPK1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

aa1-6 deleted as a result of cloning strategy

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Publication

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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