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We narrowed to 9,184 results for: Pol;

Showing: 9031 - 9060 of 9184 results

  1. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85

    Plasmid
    #111736
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q85 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111736-plasmid-map-sequence-id-247714

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  2. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

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  3. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

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  6. MKC89_CCR5(UbC-tEPOR-2A-YFP)

    Plasmid
    #232404
    Purpose
    AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer, Matthew Porteus
    Article
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232404-plasmid-map-sequence-id-462769

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  7. pCMVtrunc LifeactΔC4-msfGFPΔN7

    Plasmid
    #231555
    Purpose
    Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231555-plasmid-map-sequence-id-459696

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  8. pCA528 IST1 L328D,L355A (316-366)(C-Cys)

    Plasmid
    #193037
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D and L355A mutations that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, L355A, Residues 316-366, Non-native C-term…
    Promoter
    T7
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193037-plasmid-map-sequence-id-379006

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  10. pLX304-EZH1-dPSET

    Plasmid
    #203586
    Purpose
    Expresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.
    Depositor
    Abhishek Chakraborty, William Kaelin
    Article
    Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5
    Insert
    EZH1 (EZH1 Human)
    Use
    Lentiviral
    Tags
    V5-tagged
    Expression
    Mammalian
    Mutation
    deletion of aa 744-747 and partial resistance to …
    Promoter
    CMV
    Available Since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    203586-plasmid-map-sequence-id-423597

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  12. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    James Fleckenstein
    Article
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA-DUF::His8
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Available Since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    129732-plasmid-map-sequence-id-257612

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  23. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  24. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  28. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid
    #231549
    Purpose
    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    ACTB (ACTB Human)
    Tags
    msfGFP (intramolecular)
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231549-plasmid-map-sequence-id-459702

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  29. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid
    #231548
    Purpose
    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    ACTB (ACTB Human)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231548-plasmid-map-sequence-id-459700

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Showing: 9031 - 9060 of 9184 results