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CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

Plasmid

#222988

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1 - 14xUAS:CaSR-EGFP

Plasmid

#191502

Purpose

A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

Depositor

Michael Granato

Article

Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

Insert

CaSR-EGFP-SV40

Use

Creation of transgenic fish using tol1 transgenes…

Promoter

14X UAS

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLPP4

Plasmid

#209966

Purpose

Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P40)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLP-EV

Plasmid

#209973

Purpose

Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

CDS (mock)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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GB4665

Plasmid

#215270

Purpose

Module for the expression of nptII and the N. nambi LUZ and CPH genes flanked by insulators on the sides of the construction.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

MAR10-SF-nptII-LUZ-CPH-MAR10-SF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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RT-4

Plasmid

#220443

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (PUF3 Budding Yeast)

Tags

3xHA

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-KRf-TetR

Plasmid

#216269

Purpose

Construct 2: Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Lipid binding motif (KRf) - TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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MGS0156_pET29b

Plasmid

#215410

Purpose

Expression construct for MGS0156 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MGS0156

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPT1

Plasmid

#209995

Purpose

Contains Level 0 Part: Terminator (TB1006) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

Terminator (TB1006)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLPS1

Plasmid

#209992

Purpose

Contains Level 0 Part: Ribosome binding site (RB0034) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

RBS (RB0034)

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACBE-ASR (pXK-607)

Plasmid

#208822

Purpose

Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Promoter

CMV, U6

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pACBE-FSR (pXK-1648)

Plasmid

#208821

Purpose

Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Promoter

CMV, U6

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE_mTurquoise2

Plasmid

#202155

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_e-4

Plasmid

#202099

Purpose

Receiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_e-3

Plasmid

#202098

Purpose

Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHIT318

Plasmid

#204508

Purpose

rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

rpl-21p::2xHA::C04F12.1 (C. elegans)

Tags

2xHA

Expression

Worm

Promoter

rpl-21 (C. elegans)

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT246

Plasmid

#204507

Purpose

csr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

2xFLAG::csr-1 (C. elegans) (csr-1 Nematode)

Tags

2xFLAG

Expression

Worm

Promoter

csr-1 (C. elegans)

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3479

Plasmid

#190417

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt

Expression

Plant

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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