We narrowed to 14,028 results for: RING

1. pSL1142 (pSPIN, pBBR1 backbone)

   Plasmid

   #160730

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
   

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

   Plasmid

   #159809

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF3-ultraID

   Plasmid

   #172878

   Purpose

   expresses ultraID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCas9-miniSdd6-PBE

   Plasmid

   #204858

   Purpose

   For cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

   Plasmid

   #84469

   Purpose

   pAAV-RAM-EGFP

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. eDrIR1

    Plasmid

    #183998

    Purpose

    Optically controlled eDrIR1 expression in mammalian cells

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrIR1 (INSR Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnCas9-miniSdd7-PBE

    Plasmid

    #204859

    Purpose

    For cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A in SpCas9

    Promoter

    ZmUbi-1

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATP1A1_G8_Dual_sgRNA

    Plasmid

    #178104

    Purpose

    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 sgRNA + user-specified sgRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hsyn-GRAB_DA2m

    Plasmid

    #140553

    Purpose

    Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2m (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBI-CMV1-mir451-CMV2-mir1

    Plasmid

    #164635

    Purpose

    Express miR-451 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR451a (MIR451A Human)
    miR-1-1 (MIR1-1 )
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Vim-mCh-FKBP

    Plasmid

    #240423

    Purpose

    Tagged vimentin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Vim-mCh-SspB

    Plasmid

    #240421

    Purpose

    Tagged vimentin for optogenetic vimentin pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

    Insert

    Vimentin (VIM Human)
    

    Tags

    SspB and mCherry

    Expression

    Mammalian

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-BaLgp120-8his

    Plasmid

    #100925

    Purpose

    Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAX-EGFP-CIP4

    Plasmid

    #178363

    Purpose

    Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Cdc42 Interacting Protein 4 (TRIP10 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI neo-hEST2

    Plasmid

    #1781

    Depositor

    Bob Weinberg

    Article

    Meyerson et al Cell 1997 Aug 22;90(4):785-95.

    Insert

    hTERT (TERT Human)
    

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pK18msB

    Plasmid

    #177839

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

    Depositor

    Christopher Johnson

    Article

    Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3 Flag beta-2-adrenergic-receptor

    Plasmid

    #14697

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B2AR (ADRB2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Expression

    Mammalian

    Promoter

    CAG and Human U6

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. ABE9

    Plasmid

    #194208

    Purpose

    Mammalian expression of ABE9

    Depositor

    Dali Li

    Article

    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

    Insert

    TadA-8e (N108Q/L145T)-nSpCas9
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-hSyn-mScarlet

    Plasmid

    #131001

    Purpose

    AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Cristina Montagna, Elias Spiliotis

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pENTR E2 HSPter-35Sp-mito

    Plasmid

    #158727

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    A terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
    

    Use

    Other

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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