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pSCT149: pHelper(AjTn6022)_ΔTnsA

Plasmid

#200852

Purpose

Inducible expression of AjTn6022 proteins (except TnsA).

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT150: pHelper(AjTn6022)_ΔTnsB

Plasmid

#200853

Purpose

Inducible expression of AjTn6022 proteins (except TnsB).

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP124: pDonor(CyCAST)_0.5kb-cargo

Plasmid

#200848

Purpose

CyCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP116: pHelper(CyCAST)_PSP1

Plasmid

#200840

Purpose

Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP122: pHelper(CyCAST)_Shuffled-spacer

Plasmid

#200841

Purpose

Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13236

Plasmid

#219909

Purpose

The base plasmid of TUNEYALI for TF46

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46

Expression

Yeast

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(E502Q)

Plasmid

#226973

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(E502Q)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

E502Q

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Publication

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392A)

Plasmid

#226980

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392A

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(W392F)

Plasmid

#226979

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392F)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392F

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(F401A)

Plasmid

#226978

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(F401A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

F401A

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(L616E)

Plasmid

#226977

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(L616E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

L616E

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226K)

Plasmid

#226976

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226K)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226K

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(R226M)

Plasmid

#226975

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226M)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226M

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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