Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 40,900 results for: Eras

Clear keywords
Showing: 9151 - 9180 of 40900 results

  1. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid
    #105805
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Venus
    Expression
    Mammalian
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105805-plasmid-map-sequence-id-207906

    Enlarge

  2. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

    Enlarge

  6. ptdTHC

    Plasmid
    #112617
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112617-plasmid-map-sequence-id-224553

    Enlarge

  8. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169184
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169184-plasmid-map-sequence-id-333981

    Enlarge

  9. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169183
    Purpose
    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag (nsp12 C-terminus) and His6 (as internal t…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169183-plasmid-map-sequence-id-333983

    Enlarge

  11. Venus1-DeltaCT-STAT3

    Plasmid
    #123178
    Purpose
    Expresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Article
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Deletion of the SH2 and TAD domains (C-terminus, …
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123178-plasmid-map-sequence-id-238649

    Enlarge

  13. pVHC

    Plasmid
    #112614
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112614-plasmid-map-sequence-id-224545

    Enlarge

  18. pIS1 mIL6R

    Plasmid
    #60797
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60797-plasmid-map-sequence-id-97808

    Enlarge

  19. pGG369

    Plasmid
    #165606
    Purpose
    Vector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    ω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…
    Expression
    Bacterial
    Mutation
    Recoding of BsaI site in the AmpR cassette, two B…
    Promoter
    lacUV2 driving ω-dCas9 and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165606-plasmid-map-sequence-id-330964

    Enlarge

  20. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169181
    Purpose
    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    No purification tag and nsp7-nsp8 fusion protein …
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169181-plasmid-map-sequence-id-334012

    Enlarge

  22. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

    Enlarge

  24. pmTHC

    Plasmid
    #112616
    Purpose
    promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Promoterless vector, ready for promoter for mamma…
    Tags
    mTeal (mTFP1)
    Expression
    Mammalian
    Promoter
    No Promoter
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    112616-plasmid-map-sequence-id-224548

    Enlarge

  25. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

    Enlarge

Showing: 9151 - 9180 of 40900 results