We narrowed to 6,639 results for: itch
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Plasmid#160439PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.DepositorAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3-CBX1mut-Dronpa
Plasmid#138250PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-Dronpa
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR11
Plasmid#69154Purposeread-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IVDepositorInsertsUseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterfzr-1 and rps-27Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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GFP-itis pBE-t
Plasmid#195342PurposeBase editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110VDepositorInsertsecTadA(8e)-SpCas9-NG
gRNA ctctacgcgggtcttgtagt
UseCRISPRMutationSpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…PromoterLac and ProCAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
MYR-FR-C2D-EGFR
Plasmid#179262PurposeLight-induced clustering of EGFR cytosolic domainsDepositorInsertEGFR (EGFR Human)
UseLentiviralTagsCry2 PHR, FUS, FusionRed, and Myristoylation sequ…PromoterSFFVAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-LoxP-DsRed-LoxP-eGFP
Plasmid#65726PurposeFunctions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.DepositorInsertLoxP-DsRed-LoxP-eGFP
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mCherry RGS4(del 1-33) in pcDNA3.1
Plasmid#64207PurposemCherry fused between Cry2 and RGS4(del 1-33) to study cell migrationDepositorTagsmcherryExpressionMammalianPromoterCMVAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-TeLC-P2A-dTomato
Plasmid#159102PurposeCre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.DepositorInsertTeLC-P2A-NLS-dTomato
UseAAVPromoterhSynapsinAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG17) PAGER(TF)
Plasmid#229997PurposeExpresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-biPAC
Plasmid#169127PurposeAAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPACDepositorInsertmKate2-biPAC
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationcodon optimized to mousePromoterEF1aAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CIBN-CAAX
Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorInsertCIBN (CIB1 Mustard Weed)
TagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianPromoterSFFVAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAP05
Plasmid#186261PurposesfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoterDepositorInsertsExpressionBacterialPromoterBBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PR_GEF (2XPDZ-mCherry-Larg (DH)
Plasmid#80407PurposeRecruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cellsDepositorInsert2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)
TagsmCherryExpressionMammalianMutationDH domain only (aa 766-997)PromoterCMVAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX
Plasmid#188994PurposeA plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
TagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…PromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hCaspase-9
Plasmid#208787PurposeInducibly expresses human Caspase 8 fused to light-activatable Cry2DepositorAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-PDL1(KN035) PAGER(TF)
Plasmid#230003PurposeExpresses anti-PDL1 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-KN035-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-TIAM1-DHPH
Plasmid#176127PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseLentiviralAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
Plasmid#171615PurposeSoma-targeted Cal-Light with KA2DepositorInsertTM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
UseSynthetic BiologyTagsMycExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only