167,212 results

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pLV_3xFLAG-ATP6V0A3-mScarlet

Plasmid

#228867

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG and mScarlet

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-zSpRY

Plasmid

#179317

Purpose

In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

Depositor

Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Zebrafish codon-optimized Cas9 variant SpRY

Use

CRISPR; In vitro transcription by t3 promoter

Tags

SV40-NLS

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-mmA1

Plasmid

#61090

Purpose

expression of fusion protein: MBP-hnRNP A1

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

hnRNP A1 (Hnrnpa1 Mouse)

Tags

H6_MBP

Expression

Bacterial

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3

Plasmid

#44727

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

Maternally Expressed Gene 3 (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-F1888L

Plasmid

#69010

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-F1888L (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Phenylalanine 1888 to Leucine

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO-GRAB-gDA3m

Plasmid

#208707

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons in a cre-dependent manner

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Article

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Tags

Strep(II)-tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-M2

Plasmid

#44170

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Insert

Influenza A virus M2 matrix gene

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGLS_2

Plasmid

#163459

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting GLS (GLS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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ssFv_lenti_wtATMSPARK2

Plasmid

#195249

Purpose

express wtATMSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

ATMSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ssfv

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

Plasmid

#176531

Purpose

PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

KGV transcription factor
TagBFP

Use

Piggybac transposase integration vector

Tags

ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

Expression

Mammalian

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-Zim3-MeCP2

Plasmid

#220143

Purpose

doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

Depositor

Frank Buchholz

Article

Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

Insert

dCas9-Zim3-MeCP2

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

Tre-3G

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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GST-RBD

Plasmid

#15247

Depositor

Martin Schwartz

Article

Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

Insert

Rhotekin (Rtkn Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 7-89 (Rho binding domain)

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

Plasmid

#221360

Purpose

Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

Depositor

Institute for Protein Innovation (IPI)

Insert

Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-MT GLI2 delta N

Plasmid

#17649

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Mutation

Missing 328 N-terminal amino acids

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pET MBP mCherry LIC cloning vector (MBP-mCherry)

Plasmid

#29747

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

Expression

Bacterial

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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