162,109 results
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Plasmid#128608PurposeExpresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAS:mCherry
Plasmid#170994PurposeUAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.DepositorInsertUAS:mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterSynthetic 14x UAS:Gal4Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3.3 N-MYC TIP60
Plasmid#213003Purposeexpresses human TIP60DepositorInsertKAT5 (KAT5 Human)
UseTagsMYCExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLychee
Plasmid#219400PurposeConstitutive expression of mLychee in E. coliDepositorInsertmLychee
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NLS-actin-R62D
Plasmid#118381PurposeGateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationNLS and R62DPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I tension sensor (F40-based)
Plasmid#119186PurposeThe F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcGHLT-B-DDB1
Plasmid#48638PurposeExpresses Full Lenght DDB1 in Insect Cells (ampR)DepositorInsertDDB1 (DDB1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-RIGI-Apex- T2A-EGFP
Plasmid#167288PurposeLentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.DepositorInsertDDX58 (RIGI Human)
UseLentiviralTagsAPEX2 and EGFPExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pcDNA3
Plasmid#188447PurposeB-gTEMP for mammalian expressionDepositorInsertB-gTEMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FusionRed-Fascin-C-10
Plasmid#56117PurposeLocalization: Cytoskeleton, Excitation: 580, Emission: 608DepositorInsertFascin (FSCN1 Human)
UseTagsFusionRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti CMV Blast DEST (706-1)
Plasmid#17451Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, BlastDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_deltaUBA_UBAP2L_mCherry
Plasmid#105287Purposepuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2LDepositorInsertUBAP2L (UBAP2L Human)
UseLentiviralTagsmCherryExpressionMammalianMutationdeleted amino acids 20-108PromoterTRE promoter, Tet ONAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-NDP52
Plasmid#188785PurposeFor mammalian expression of NDP52 tagged with mEGFPDepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLemon
Plasmid#219399PurposeConstitutive expression of mLemonin E. coliDepositorInsertmLemon
UseTagsExpressionBacterialMutationPromoterproDpAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-Tyr
Plasmid#113644PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.DepositorInsertMjYRS and tRNA gene
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits