Addgene

We narrowed to 1,846 results for: Ipp

Showing: 901 - 930 of 1846 results

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-TEV-TBP6.9F34MF37MF77M

Plasmid

#168268

Purpose

Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

Depositor

Joseph Wedekind

Article

Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

Insert

TBP6.9

Use

Tags

6xHis tag N-terminus, TEV protease cleavage site

Expression

Bacterial

Mutation

changed F34M, F37M and F77M

Promoter

T7 promotor

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11A(E186Q)-HA

Plasmid

#209249

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E186Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124434

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Use

Tags

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1

Plasmid

#172587

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP7

Plasmid

#178004

Purpose

Expresses N-terminally mEGFP-tagged PARP7

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP7 (TIPARP Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Adeno EA

Plasmid

#64071

Purpose

Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Insert

U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9

Use

Adenoviral

Tags

FLAG-Cas9

Expression

Mutation

Promoter

U6 and CBh

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

Plasmid

#170229

Purpose

Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Q64L

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMx/ATP9B-HA

Plasmid

#209231

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9B (ATP9B Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1

Plasmid

#39281

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1

Plasmid

#39282

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Promoter

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Use

Tags

octahistidine tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-EGFP:LIMD1

Plasmid

#108230

Purpose

Lentiviral vector for Tet-inducible LIMD1 expression

Depositor

Kenneth Irvine

Article

Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

Insert

LIMD1 (LIMD1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Tet/ON Minimal CMV promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_18xTEAD_luc2

Plasmid

#173003

Purpose

firefly luciferase reporter vector with 18x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

18xTEAD-luc2

Use

AAV and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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011LP-pAAV-TNT4-EmGFP-pA

Plasmid

#120280

Purpose

AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

Depositor

Xander Wehrens

Article

Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

Insert

EmGFP

Use

AAV

Tags

3XFlag and HA

Expression

Mutation

Promoter

TNT Cardiac Troponin T promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt-3HA

Plasmid

#39284

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 4bp deletion (TA…

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1-3HA

Plasmid

#39285

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Promoter

Available since

Aug. 14, 2012

Availability

Academic Institutions and Nonprofits only
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194284

Purpose

EF1a-dCas9-KRAB-GFP with nontarget sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1-GFP

Plasmid

#41616

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-STK4

Plasmid

#172989

Purpose

constitutive expression of FLAG-tagged STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-STK4 (STK4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP6

Plasmid

#178005

Purpose

Expresses N-terminally mEGFP-tagged PARP6

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP6 (PARP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP15

Plasmid

#178000

Purpose

Expresses N-terminally mEGFP-tagged PARP15

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP15 (PARP15 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRosa26-GT

Plasmid

#40025

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-LATS2-KR

Plasmid

#33100

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

lats2 (Lats2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

K655R, Kinase Dead

Promoter

CMV

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36880

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

tTA2
tdT-3Myc
insulator

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-TAOK3

Plasmid

#172993

Purpose

constitutive expression of FLAG-tagged TAOK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-TAOK3 (TAOK3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Nrxn3α-AS4-

Plasmid

#184332

Purpose

Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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