We narrowed to 12,157 results for: pcdna

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pcDNA3.1(+)-deltaFlincG

Plasmid

#19734

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

delta-FlincG

Tags

His

Expression

Mammalian

Mutation

aa 77-356

Available Since

March 26, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Tags

Citrine and ECFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-dnCDK5

Plasmid

#1344

Depositor

Li-Huei Tsai

Insert

dnCDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Mutation

D144N

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3/hArf3(WT)-HA

Plasmid

#79414

Purpose

Expresses C-terminally HA-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (ARF3 Human)

Tags

HA

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hUCP4-NE

Plasmid

#102362

Purpose

Overexpression of solute carrier family 25 member 27 in mammalian cells

Depositor

Shu Leong Ho

Insert

solute carrier family 25 member 27 (SLC25A27 Human)

Tags

NE

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pcDNA3

Plasmid

#206069

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR(T/A)

Plasmid

#118471

Purpose

Negative-control mutant for blueAKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR

Plasmid

#118472

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_300

Plasmid

#124095

Purpose

orange calcium-modulated bioluminescent indicator, 300nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 300

Expression

Mammalian

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-T7-Keap1

Plasmid

#21552

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

kelch-like ECH-associated protein 1 (KEAP1 Human)

Tags

T7

Expression

Mammalian

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pcDNA3

Plasmid

#206072

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hL1

Plasmid

#89411

Purpose

mammalian expression of hL1

Depositor

Fritz Rathjen

Article

De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CARMA1

Plasmid

#44431

Depositor

Xin Lin

Insert

CARMA1 (CARD11 Human)

Expression

Mammalian

Mutation

See depositor comments below.

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R4-uAb

Plasmid

#101800

Purpose

Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

Depositor

Matthew DeLisa

Article

Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

Insert

R4-uAb

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:VPR

Plasmid

#135983

Purpose

Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the VPR activation domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-p48

Plasmid

#11614

Depositor

Steven Johnson

Insert

Interferon-Stimulated Gene Factor 3 (IRF9 Human)

Tags

HA

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3.1neo-14-3-3-CLuc

Plasmid

#107611

Purpose

measure LATS activity by Luciferase Assay

Depositor

Xiaolong Yang

Article

Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

Insert

pCDNA3.1hygro-BamHI-CLuc1433-NotI

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F2

Plasmid

#236432

Purpose

transient overexpression of E2F2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-S-Luc_cyto

Plasmid

#113926

Purpose

Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAGEdeltaCyto

Plasmid

#71436

Purpose

mammalian expression of human RAGE cytoplasmic domain mutant

Depositor

Henri Huttunen

Article

Huttunen et al J Biol Chem. 1999 Jul 9;274(28):19919-24

Insert

RAGE (AGER Human)

Expression

Mammalian

Mutation

delta 367-404

Promoter

CMV

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-CaNAR2

Plasmid

#64732

Purpose

Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-CaNAR2

Tags

Cerulean3, N-terminal 27 amino acids from CYP450,…

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-V5-hTEV

Plasmid

#65800

Purpose

expresses human codon-optimised TEV protease

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

V5-human TEV

Tags

V5

Expression

Mammalian

Mutation

codon-optimised to express in mammalian cells

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-MKK1K97A

Plasmid

#107974

Purpose

expression of a dominant negative MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 K97A (MAP2K1 Human)

Expression

Mammalian

Mutation

dominant negative; see Depositor Comments below

Promoter

CMV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Plagl2

Plasmid

#228061

Purpose

transiently express mouse Plagl2

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Plagl2 (Plagl2 Mouse)

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-HA

Plasmid

#79427

Purpose

Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

Insert

Arf5 (ARF5 Human)

Tags

HA

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-OPN-V5

Plasmid

#11617

Depositor

Steven Johnson

Insert

Osteopontin (SPP1 Human)

Tags

His and V5

Expression

Mammalian

Mutation

C9L and E160K

Promoter

CMV

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-TRAIL-R1

Plasmid

#61382

Purpose

Expresses HA tagged TRAIL receptor 1

Depositor

Francis Chan

Article

Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

Insert

TRAIL receptor 1

Tags

HA and TNF receptor leader peptide

Expression

Mammalian

Mutation

TRAIL-R1 leader peptide (aa1-23) replaced with TN…

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
Twitch-4 pcDNA3

Plasmid

#49533

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-4

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only