We narrowed to 5,218 results for: Ado

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tol2-GFAP::CD14

Plasmid

#59593

Purpose

Magnetic cell sorting of ES cell-derived astrocytes

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

human CD14 (CD14 Human)

Expression

Mammalian

Promoter

gfa2

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-Climp63(1–336)

Plasmid

#186957

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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BPTF PHD and Bromodomain

Plasmid

#92101

Purpose

Expresses GST fusion PHD and Bromodomain from BPTF protein

Depositor

Albert Jeltsch

Article

Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

Insert

PHD and Bromodomain (BPTF Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_SRY_P2A_Hygro_Barcode

Plasmid

#120485

Purpose

Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SRY (SRY Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_1

Plasmid

#101076

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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FAM177A1isoform2-GFP

Plasmid

#224589

Purpose

For expression of FAM177A1-GFP in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_KDM1A_1

Plasmid

#86297

Purpose

Encodes gRNA for 3' target of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM1A (KDM1A Human)

Use

CRISPR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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2xCBX7CD_W35A-IFP2.0C

Plasmid

#215755

Purpose

Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xCBX7CD_W35A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PX458_KDM1A_2

Plasmid

#86298

Purpose

Encodes gRNA for 3' target of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM1A (KDM1A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_SOX3_P2A_Hygro_Barcode

Plasmid

#120481

Purpose

Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SOX3 (SOX3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAC84-pCR8-dCas9

Plasmid

#48218

Purpose

dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A)

Use

CRISPR and Gateway Cloning

Tags

HA Tag

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFETCh_CEBPA

Plasmid

#86293

Purpose

Donor vector for 3' FLAG tag of human CEBPA

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CEBPA homology arms (CEBPA Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD-IFP2.0C

Plasmid

#215750

Purpose

Expresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD_W42A-IFP2.0C

Plasmid

#215751

Purpose

Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD_W42A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-Strep/HA

Plasmid

#124666

Purpose

Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_MYOG_P2A_Hygro_Barcode

Plasmid

#120465

Purpose

Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYOG (MYOG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

Plasmid

#129513

Purpose

Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 C88S (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(276-598)

Plasmid

#135437

Purpose

Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Splicing Factor Proline And Glutamine Rich (SFPQ Human)

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_1

Plasmid

#86307

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

Plasmid

#194283

Purpose

EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

Depositor

Elizabeth Kirby

Article

Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

Insert

dCas9-KRAB-GFP

Use

CRISPR and Lentiviral

Tags

GFP

Promoter

EF1a

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_ARID5B

Plasmid

#86288

Purpose

Donor vector for 3' FLAG tag of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID5B homology arms (ARID5B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-C-CARMIL2b

Plasmid

#118741

Purpose

To express CARMIL2 with GFP tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Climp63(1–192)

Plasmid

#186955

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ Zf cdh1-deltaCyot-GFP

Plasmid

#185412

Purpose

GFP tagged zebrafish cdh1 lacking the cytoplasmic domain.

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

cdh1 (cdh1 Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Mutation

Cytoplasmic domain deleted

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KDM6A_2

Plasmid

#86308

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_MIER3

Plasmid

#86274

Purpose

Donor vector for 3' FLAG tag of human MIER3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MIER3 homology arms (MIER3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_POU1F1_P2A_Hygro_Barcode

Plasmid

#120473

Purpose

Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU1F1 (POU1F1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
N-TAP A2A in pcDNA3

Plasmid

#67850

Purpose

mammalian expression of A2A receptor with N terminal TAP tag

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pFETCh_KAT7

Plasmid

#86266

Purpose

Donor vector for 3' FLAG tag of human KAT7

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KAT7 homology arms (KAT7 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_FOXP1_1

Plasmid

#86299

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125236

Purpose

Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXNPHM3-Nb_MsbA#1

Plasmid

#186428

Purpose

Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

Depositor

Markus Seeger

Article

Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

Insert

Nanobody Nb_MsbA#1

Use

Affinity Reagent/ Antibody

Tags

3C cleavage site, His Tag, Maltose Binding Protei…

Promoter

pBAD

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_FOXP1_2

Plasmid

#86300

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_CEBPG

Plasmid

#86285

Purpose

Donor vector for 3' FLAG tag of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CEBPG homology arms (CEBPG Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNCX2-Orai1

Plasmid

#89818

Purpose

Express Orai1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::hGluc

Plasmid

#186768

Purpose

An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_GATAD2A

Plasmid

#86279

Purpose

Donor vector for 3' FLAG tag of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD2A homology arms (GATAD2A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BRD4 CRISPRi plasmid

Plasmid

#154890

Purpose

Human BRD4 CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

BRD4-targeting gRNA

Use

CRISPR and Lentiviral

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 PA-mCit

Plasmid

#113443

Purpose

ProteinA-mCitrine control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER.retro.puro-shMePCE_shLARP7

Plasmid

#113538

Purpose

Retroviral vector designed to knock down MePCE and LARP7 simultaneously.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE and shLARP7 (MEPCE Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_MED13_iso1

Plasmid

#135733

Purpose

Donor vector for 3' FLAG tag of human MED13_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MED13_iso1 Homology arms (MED13 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 5,218 results for: Ado