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We narrowed to 1,586 results for: Tef

Showing: 1501 - 1550 of 1586 results

  4. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Use
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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  11. FRT_htr2c(c)-GFP

    Plasmid
    #79677
    Purpose
    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP
    Depositor
    Stefan Stamm
    Article
    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do
    Insert
    serotonin receptor 2C (HTR2C Human)
    Use
    Tags
    EGFP in Frame
    Expression
    Mammalian
    Mutation
    splice site of exon Vb in consensus
    Promoter
    CMV
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  13. pMH-SFB-USP7

    Plasmid
    #99393
    Purpose
    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Insert
    USP7 (USP7 Human)
    Use
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99393-plasmid-map-sequence-id-190612

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  17. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Use
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Use
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  19. pcDNA3.1_ZKSCAN1_Tau7-12WT

    Plasmid
    #194166
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 7-12 WT
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194166-plasmid-map-sequence-id-381821

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  20. pcDNA3.1_ZKSCAN1_Tau10-12WT

    Plasmid
    #194163
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
    Use
    Tags
    3X flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194163-plasmid-map-sequence-id-381804

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  23. pcDNA3.1_Tau Authentic Alu 10-12WT

    Plasmid
    #194167
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194167-plasmid-map-sequence-id-381798

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  24. pcDNA3.1_Tau Authentic Alu 7-12WT

    Plasmid
    #194170
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    microtubule-associated protein tau 7-12 WT
    Use
    Tags
    3x Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194170-plasmid-map-sequence-id-381818

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  25. pcDNA3.1_ZKSCAN1_Tau10-12V337M

    Plasmid
    #194165
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau V337M mutation. Expresses the tau circRNA 12-->10 V337M with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 V337M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Valine 337 to Methinonine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194165-plasmid-map-sequence-id-381806

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  27. pOGS539_haPD-1

    Plasmid
    #226716
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)
    Depositor
    Gautam Dantas
    Article
    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2
    Insert
    High affinity PD-1 microbody
    Use
    Tags
    HA tag and Mating factor alpha secretion signal
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226716-plasmid-map-sequence-id-455452

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  28. pOGS539_rPD-L1nb

    Plasmid
    #226717
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)
    Depositor
    Gautam Dantas
    Article
    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2
    Insert
    Recombinant PD-L1 nanobody
    Use
    Tags
    HA tag, Histidine tag, and Mating factor alpha se…
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226717-plasmid-map-sequence-id-455449

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  30. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    70763-plasmid-map-sequence-id-240922

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  35. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid
    #194169
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 V337M
    Use
    Tags
    3X flag
    Expression
    Mammalian
    Mutation
    Changed Valine 337 to Methinonine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194169-plasmid-map-sequence-id-381800

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  43. pcDNA3.1_Tau Authentic Alu 10-12K317M

    Plasmid
    #194168
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 K317M
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Lysine 317 to Methionine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194168-plasmid-map-sequence-id-381799

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Showing: 1501 - 1550 of 1586 results