We narrowed to 62,913 results for: cloning

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pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Publication

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pEF1a-hMLH1dn

Plasmid

#174824

Purpose

Mammalian expression of human MLH1dn (codon optimized)

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

Plasmid

#53959

Purpose

allows Cre-mediated expression of transgene

Depositor

Jeffrey Robbins

Publication

Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

Type

Empty backbone

Use

Cre/Lox

Tags

Lox-CAT-Lox

Expression

Mammalian

Promoter

CMV enhancer, chicken actin promoter

Available since

June 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET-NT*-HRV3CP

Plasmid

#162795

Purpose

Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

Depositor

Gottfried Otting

Publication

Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

Insert

Human rhinovirus 14 3C protease

Tags

NT* solubility tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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MP6

Plasmid

#69669

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1

Expression

Bacterial

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pCR4-24XMS2SL-stable

Plasmid

#31865

Depositor

Robert Singer

Publication

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

24xMS2 Stem Loops - Stable

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

45 Articles

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pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Publication

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

32 Articles

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Publication

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

Plasmid

#119816

Purpose

3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

Depositor

Ethan Abel

Publication

Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

Inserts

Luciferase
Puromycin

Use

Lentiviral and Luciferase

Tags

EGFP and V5

Expression

Mammalian

Mutation

Stop codon removed in the frame of the V5 frame v…

Promoter

CMV/TO and Human Phosphoglycerate kinase

Available since

Jan. 2, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

14 Articles

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pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

Plasmid

#32702

Purpose

Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

Depositor

Hans Clevers

Publication

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Insert

eGFP

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available since

Dec. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pMOD_C3012

Plasmid

#91099

Purpose

Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi1

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Tags

6xHis tag and NLS

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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Cre Reporter

Plasmid

#62732

Purpose

Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

LoxP-DsRED-STOP-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Tags

LoxP sites

Expression

Mammalian

Promoter

EF1-Alpha

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pSIJ8

Plasmid

#68122

Purpose

Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

Depositor

Alex Nielsen

Publication

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Inserts

flp recombinase
rhaS
rhaR

Expression

Bacterial

Promoter

rhamnose

Available since

Dec. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

133 Articles

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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FUW mCherry-GFP-LC3

Plasmid

#110060

Purpose

To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

Depositor

Anne Brunet

Publication

Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

Insert

mCherry-GFP-LC3

Use

Lentiviral

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

39 Articles

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Lenti-luciferase-P2A-Neo

Plasmid

#105621

Purpose

3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

Depositor

Christopher Vakoc

Publication

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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