We narrowed to 9,388 results for: Coli

1. pET-6xHis-GFP(+30)

   Plasmid

   #199164

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+30)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1C3-Lux

   Plasmid

   #109383

   Purpose

   Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-LUX operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29b-pduCDE

   Plasmid

   #110138

   Purpose

   Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

   Insert

   pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC25

   Plasmid

   #177319

   Purpose

   Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

   Depositor

   Rafal Tomecki

   Article

   Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

   Insert

   TtCutA 240-603
   

   Use

   Tags

   6xHis-SUMOTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPdX

   Plasmid

   #85084

   Purpose

   Expresses putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 73 to Serine and Cysteine 85 to …

   Promoter

   T7 promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEVA221pmCDA1-T7RNAP

   Plasmid

   #167978

   Purpose

   Expresses fusion protein pmCDA1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   pmCDA1-T7RNAP (T7p07 Synthetic)
   

   Use

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-BE1

   Plasmid

   #73018

   Purpose

   Expresses BE1 with N-terminal His6 tag in E. Coli

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH1-SaSrtA

   Plasmid

   #64973

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   T7 promoter

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. His SUMO TEV SPOP 1-374

    Plasmid

    #197015

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTXRE5-Z

    Plasmid

    #112509

    Purpose

    Reporter plasmid, responsive to activated Ah receptor/Arnt

    Depositor

    Charles Miller

    Article

    Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

    Insert

    RW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    5 XREs+ minimal Cytochrome C

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-dCas9/p-bacteria

    Plasmid

    #64325

    Purpose

    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR; Tetracycline-inducible

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    pLtetO-1

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-eDHFR

    Plasmid

    #107266

    Purpose

    mCherry-eDHFR in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    mCherry-eDHFR (dhfr E.coli)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-mitoT

    Plasmid

    #174519

    Purpose

    Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

    Depositor

    Oleh Khalimonchuk

    Article

    Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

    Insert

    MitoT synthetic tether
    

    Use

    Tags

    6xHis and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. SurA

    Plasmid

    #213138

    Purpose

    Expresses chaperone SurA

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SurA (21-428) (surA E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCN34e-ktrA-yfp

    Plasmid

    #227640

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    ktrA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. His6_MBP_FXR2P_P474S_P493N

    Plasmid

    #198695

    Purpose

    Expression of human FXR2 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR2P (FXR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Changed proline 474 to serine and proline 493 asp…

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Use

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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