We narrowed to 9,849 results for: EPO

1. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

   Plasmid

   #84446

   Purpose

   AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Has Service

   AAV1, AAV5, and AAV8

   Insert

   Jaws-KGC-tdTomato-ER2
   

   Use

   AAV

   Tags

   ER2, KGC, and tdTomato

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CAG

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1 R62D actin-3XNLS P2A mCherry

   Plasmid

   #58477

   Purpose

   Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Arginine 62 to Aspartic Acid

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.hSyn.iGluSnFR3.v857.GPI

   Plasmid

   #178331

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Has Service

   AAV1

   Insert

   pAAV hSyn iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-mFgf21pro1-luc

   Plasmid

   #101797

   Purpose

   Reporter for mouse Fgf21 promoter activity

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   Fibroblast growth factor 21 promoter (Fgf21 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-MYO5C

   Plasmid

   #135404

   Purpose

   Expresses GFP-tagged human MYO5C in mammalian cells.

   Depositor

   Richard Cheney

   Article

   Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

   Insert

   MYO5C (MYO5C Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Relative to the GenBank MYO5C cDNA sequence repor…

   Promoter

   CMV

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX304-GW-DCK*-IRES-GFP

   Plasmid

   #176291

   Purpose

   Gateway vector for use in generating POI-DCK* fusion reporter

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Insert

   Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S74E R104M D133A

   Promoter

   CMV

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.22-PGK1-HRE::dLUC

   Plasmid

   #128095

   Purpose

   Hypoxia response element real-time luciferase reporter

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
   

   Use

   Luciferase

   Tags

   drives expression of destabilized luciferase (2CP…

   Expression

   Mammalian

   Mutation

   Promoter

   inserted PGK1 hypoxia response elements (HRE) (x3)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

   Plasmid

   #67986

   Purpose

   Cas9 activity reporter with mCherry and BFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Inserts

   U6gRNA cassette, PGKmCherryABFP cassette, WPRE
   Guide RNA targeting modified BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV.GFAP.iGluSnFR3.v857.SGZ

   Plasmid

   #178337

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV GFAP iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CAG-FLEX-rc [Chronos-tdTomato]

    Plasmid

    #84484

    Purpose

    AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSyn.iGluSnFR3.v857.IgK-NGR

    Plasmid

    #196227

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.NGR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and NGR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.CAG.FLEX.iGluSnFR3.v857.GPI

    Plasmid

    #196218

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG.FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX304-DCK*-GW-IRES-GFP

    Plasmid

    #176289

    Purpose

    Gateway vector for use in generating DCK*-POI fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

    Plasmid

    #128327

    Purpose

    Reporter to evaluate YAP1/TEAD-mediated gene transcription

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Inserts

    H2B mCherry
    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.GFAP.iGluSnFR3.v857.GPI

    Plasmid

    #178338

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-ACE2-his expression plasmid

    Plasmid

    #158089

    Purpose

    Expresses ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCAR_Cas9-hygro_GFP

    Plasmid

    #162075

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.hSyn.iGluSnFR3.v857.PDGFR

    Plasmid

    #178329

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

    Plasmid

    #196219

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV2

    Insert

    pAAV CAG FLEX iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV Marina-T2A-nls-mCherry

    Plasmid

    #74216

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179432

    Purpose

    Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    HS4-TRE-cit-HS4-mch (DH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

    Plasmid

    #190182

    Purpose

    Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    iRFP
    hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
    iRFP
    hGeminin(1/110)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV.GFAP.iGluSnFR3.v857.PDGFR

    Plasmid

    #178336

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUSE-rIgG-Fc2-ICAM1

    Plasmid

    #156462

    Purpose

    Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

    Depositor

    Dirk Mielenz

    Article

    Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

    Insert

    Intercellular Adhesion Molecule 1 (Icam1 Mouse)
    

    Use

    Tags

    ICAM1-rFc

    Expression

    Bacterial and Mammalian

    Mutation

    endogenous signal peptide replaced with IL2 signa…

    Promoter

    hEF1-HTLC

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-MKK1

    Plasmid

    #107973

    Purpose

    expression of MAP Kinase Kinase

    Depositor

    Edwin Krebs

    Article

    Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

    Insert

    MKK1 (MAP2K1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid

    #216155

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic splice site and C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti_EF1a_Flag_TP53_R248W+P278A

    Plasmid

    #183115

    Purpose

    Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 278 to alanine and arginine 248 t…

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge