We narrowed to 14,011 results for: flag

1. Ubiq-Cas9.874W

   Plasmid

   #112686

   Purpose

   Express hSpCas9 under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   Ubiquitin-63E promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. GreenT-EC - Display - CyRFP1

   Plasmid

   #193943

   Purpose

   Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

   Depositor

   Oliver Griesbeck

   Article

   Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

   Insert

   GreenT-EC
   

   Use

   Tags

   Igk_secretion - HA-Flag and PDGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-UTY-F

   Plasmid

   #17439

   Purpose

   Expression of UTY in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   UTY (UTY Human)
   

   Use

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-FNLSNG-P2A-Puro

   Plasmid

   #136900

   Purpose

   Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SadCas9 VPR

   Plasmid

   #188514

   Purpose

   Expresses FLAG tagged SadCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle-FEN1hWT

   Plasmid

   #35027

   Depositor

   Sheila Stewart

   Article

   Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

   Insert

   FEN1 (FEN1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

   Plasmid

   #165429

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII0.4

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-UTY-F

   Plasmid

   #40617

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

   Insert

   UTY (UTY Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   EF1

   Available Since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pEntr-GATA4flbio

   Plasmid

   #32937

   Depositor

   William Pu

   Article

   He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

   Insert

   GATA4 (Gata4 Mouse)
   

   Use

   Gateway

   Tags

   flag-bio

   Expression

   Mutation

   Promoter

   none

   Available Since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. IRES-GFP-AXL-KD

    Plasmid

    #65498

    Purpose

    Expresses kinase dead AXL in mammalian cells with cistronic EGFP

    Depositor

    Aaron Meyer

    Article

    Meyer et al Cell Syst. 2015 Jul 29;1(1):25-36.

    Insert

    Tyrosine-protein kinase receptor UFO (AXL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K567R

    Promoter

    CMV

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-CyclinF

    Plasmid

    #32975

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Tags

    Flag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMLM3705

    Plasmid

    #47754

    Purpose

    Expresses mammalian cell codon-optimized dCas9-VP64

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    codon optimized dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9 (catalytically i…

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. BII-ChBtW-iresFUCCI

    Plasmid

    #133371

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28 Set9 wt

    Plasmid

    #24082

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Use

    Tags

    FLAG and His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA Set9 mutant

    Plasmid

    #24085

    Depositor

    Danny Reinberg

    Article

    Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

    Insert

    Set9 (SETD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H297A

    Promoter

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GreenT-EC.b - Display - CyRFP1

    Plasmid

    #193944

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TAL2451

    Plasmid

    #36840

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HMGA2-TALEN_Right (Hmga2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. GreenT-EC.c - Display - CyRFP1

    Plasmid

    #193945

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFsynW SYB2 reporter

    Plasmid

    #197276

    Purpose

    Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. LbdCas12a VPR

    Plasmid

    #188513

    Purpose

    Expresses FLAG tagged LbdCas12a VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMCS-rybozyme-IRES-CAS9

    Plasmid

    #64668

    Purpose

    Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

    Depositor

    Wataru Fujii

    Article

    Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

    Inserts

    CAS9
    HDV ribozyme
    

    Use

    CRISPR

    Tags

    Flag, Internal ribosome entry site, and guide RNA…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pN1-Lck-GCaMP3

    Plasmid

    #26974

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

    Insert

    Lck-GCaMP3
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Plasmid contains N-terminal 26 amino acid membran…

    Promoter

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-R4-uAb

    Plasmid

    #101800

    Purpose

    Mammalian cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX 4T1 hBad

    Plasmid

    #35567

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    bad (BAD Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

29. R4227X-F

    Plasmid

    #21371

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG (following D4226)

    Expression

    Mammalian

    Mutation

    Changed R4227 to stop codon

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pCS2 hSmad1-WT

    Plasmid

    #22989

    Depositor

    Edward De Robertis

    Article

    Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

    Insert

    Smad1 (SMAD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only