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pLenti 4.1 Ex miR200c-141

Plasmid

#35534

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200c-141 genomic cluster (MIR200C, MIR141 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-OGT1

Plasmid

#29760

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

OGT1 (OGT Human)

Tags

Flag

Expression

Mammalian

Mutation

N764D and K1010E

Available Since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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HA-GABARAPL2

Plasmid

#137761

Purpose

Expression of GABARAPL2

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL2 (GABARAPL2 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2 mCherry Sec61B

Plasmid

#102246

Purpose

Expresses fusion of CRY2PHR with mCherry and Sec61B

Depositor

Bianxiao Cui

Article

Che et al ACS Synth Biol. 2015 Oct 16;4(10):1124-3

Insert

CRY2PHR-mCherry-SEC61beta (CRY2 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5a-Myc

Plasmid

#186613

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5A (KIF5A Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Str-Endo-KKXX_SBP-EGFP-LIMP2

Plasmid

#222323

Purpose

Synchronize the trafficking of LIMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9::BFP-humanized

Plasmid

#44247

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 fused to BFP

Use

CRISPR and Retroviral

Tags

2xNLS & tagBFP

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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eTd-CBE

Plasmid

#196601

Purpose

Mammalian expression of eTd-CBE

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA

Plasmid

#108745

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

Plasmid

#214881

Purpose

Lentiviral vector encoding RfxCas13d targeting B2M guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crB2M-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA

Plasmid

#108746

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SLC38A9.1

Plasmid

#71855

Purpose

overexpression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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DRH296: FCK-Optopatch2

Plasmid

#51694

Purpose

Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

Optopatch2

Use

Lentiviral

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pAcBac1.tR4-AcdRS82

Plasmid

#174101

Purpose

Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

M. barkeri tRNA synthetase for acridone82

Expression

Mammalian

Mutation

N311S, C313G, V366A, W382T

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 TIFA WT

Plasmid

#231589

Purpose

TIFA WT gene under control of a doxycycline-inducible promoter

Depositor

Thomas Henry

Article

Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

Insert

TIFA (TIFA Human)

Use

Lentiviral

Tags

3X flag

Expression

Mammalian

Promoter

doxycycline

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-E-cadherin promoter

Plasmid

#61798

Purpose

Luciferase reporter containing the mouse E-cadherin promoter

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

-178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1-shQKI-3373

Plasmid

#115456

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shQKI-3373 (QKI Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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ABE8.17-m

Plasmid

#136298

Purpose

expresses ABE8.17-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.17-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCINeo-myc-Itch (C822S)/2860

Plasmid

#11428

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Tags

myc

Expression

Mammalian

Mutation

cysteine at 822 mutated to serine

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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p35sheLL

Plasmid

#40626

Depositor

Simon Beddows

Article

Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

Inserts

HPV35 L1
HPV35 L2

Expression

Mammalian

Mutation

Codon optimized for mammalian expression

Promoter

CMV and Polio IRES

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only

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HA-GABARAPL1

Plasmid

#137759

Purpose

Expression of GABARAPL1

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL1 (GABARAPL1 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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