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Addgene

We narrowed to 2,761 results for: SRS

Showing: 1241 - 1280 of 2761 results
  1. pMP71-mGFP-llo190-mc38minor

    Plasmid
    #174604
    Purpose
    expresses palmitoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1
    Depositor
    Insert
    palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pJV126

    Plasmid
    #124250
    Purpose
    In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Insert
    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pJV127

    Plasmid
    #124251
    Purpose
    In vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Insert
    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pJV128

    Plasmid
    #124252
    Purpose
    In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Insert
    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pJV129

    Plasmid
    #124253
    Purpose
    In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Insert
    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pSR357.12

    Plasmid
    #125107
    Purpose
    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115
    Depositor
    Inserts
    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    A84T - see depositor comments below and Q183K-see…
    Promoter
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    Insert
    ABE8e-NRCH
    Use
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Mutation
    Promoter
    pRhaBAD
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pMS2M-PH-gagpol-D64V

    Plasmid
    #166031
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Insert
    MS2-Gag-Pol
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Insert
    atf4 (ATF4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pMDlg/pRRE-int-MS2M

    Plasmid
    #166032
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Insert
    Gag-Pol-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pGG-B1

    Plasmid
    #78504
    Purpose
    Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid
    Inserts
    AAV2 Rep
    AAV-B1 capsid
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pLKO_AU1.C.HA_mCherry-NLS

    Plasmid
    #178209
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.HA and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pLKO_Ollas.S.V5_mCherry-NLS

    Plasmid
    #178211
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    Nuclear Localization Signal and Ollas.S.V5
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. Lenti-pNFkB::Cre-mRuby3

    Plasmid
    #164137
    Purpose
    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa
    Depositor
    Insert
    NFkB promoter-Cre-T2A-mRuby3
    Use
    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NFkB synthetic promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1280 of 2761 results