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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,278 results for: Vars;

Showing: 9661 - 9690 of 11278 results

  1. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid
    #223245
    Purpose
    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry
    Depositor
    Stavros Lomvardas
    Insert
    Mex3a (Mex3a Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    4 point mutations in RING Domain: Cys 484 Ala; Hi…
    Promoter
    CMV
    Available Since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    223245-plasmid-map-sequence-id-441774

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  5. P427-FBP_13

    Plasmid
    #139704
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139704-plasmid-map-sequence-id-296524

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  6. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Promoter
    EF-1-alpha promoter
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  10. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  11. pc DNA FRT TO- APP695

    Plasmid
    #114193
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    wild type splice variant 695: 695AA (2085bp, lack…
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114193-plasmid-map-sequence-id-224265

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  15. pcDNA3.1(+)GαoA-91-NLuc

    Plasmid
    #207151
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207151-plasmid-map-sequence-id-410283

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  16. pcDNA3.1(+)GαoB-91-NLuc

    Plasmid
    #207152
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAO
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)G
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207152-plasmid-map-sequence-id-410279

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  17. P336-FBP_11

    Plasmid
    #139702
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139702-plasmid-map-sequence-id-296517

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  23. AAV-sCAG-eGFP-ACAP1

    Plasmid
    #203800
    Purpose
    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    203800-plasmid-map-sequence-id-410892

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  24. pET30b_OmpAT7Sav

    Plasmid
    #138589
    Purpose
    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d
    Insert
    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    Use
    Synthetic Biology
    Tags
    OmpA export signal and T7 tag
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138589-plasmid-map-sequence-id-274488

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  29. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108867-plasmid-map-sequence-id-209739

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Showing: 9661 - 9690 of 11278 results