We narrowed to 11,278 results for: Vars;

1. pES134

   Plasmid

   #127551

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Gavin Thomas, Emmanuele Severi

   Article

   Wu et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   F3-flanked spectinomycin resistance cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIAA5::LUC

   Plasmid

   #141169

   Purpose

   Luminescent reporter for auxin signaling

   Depositor

   Patrick Schäfer, Vardis Ntoukakis

   Article

   Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

   Insert

   AtIAA5 promoter
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtIAA5, At1g15580

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-6xHis-GFP(+24)

   Plasmid

   #199163

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+24)
   

   Tags

   MGHHHHHHGGA

   Mutation

   E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p458 VRER

   Plasmid

   #101728

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p330 VQR

   Plasmid

   #101730

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB036d

   Plasmid

   #99216

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK, and PproA

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR1A_no_ccDB_mUty

   Plasmid

   #158570

   Purpose

   V5-tagged sequence of mUty cloned into entry vector for recombination

   Depositor

   Christian Deschepper

   Article

   Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

   Insert

   Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. P303-FBP_2

   Plasmid

   #139693

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDB036a

   Plasmid

   #99215

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK and PproD

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-ND4 Right-GSVG-UGI

    Plasmid

    #187414

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-ND4 Right-E1347A-UGI

    Plasmid

    #187415

    Purpose

    human mitochondrial DNA ND4 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    AAV and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. wt HA-dynamin 1 pUHD10-3

    Plasmid

    #34688

    Depositor

    Sandra Schmid

    Article

    Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

    Insert

    dynamin 1 (DNM1L Human)
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

13. UAS::PTPRFa.1-DN-GFP

    Plasmid

    #37110

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)
    

    Use

    Tol2 gateway expression vector

    Tags

    EGFP

    Mutation

    deleted amino acids 1326-1894

    Promoter

    UAS-E1b

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH760-CEN-mCherry_v3

    Plasmid

    #40598

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
    

    Expression

    Yeast

    Mutation

    mCherry ORF partially codon optimised for yeast e…

    Promoter

    GPD (TDH3)

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSC201

    Plasmid

    #129387

    Purpose

    A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

    Depositor

    Silvia Cardona

    Article

    Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

    Insert

    oriR6K and oriT
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    N/A

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYC121

    Plasmid

    #63917

    Purpose

    Recipient overexpressing vector to construct MYC tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    MYC

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid

    #209837

    Purpose

    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Promoter

    hEF1-HTLV promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-EGFP-DmGW182

    Plasmid

    #22419

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    gawky, variant A (gw Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

    Plasmid

    #45578

    Purpose

    Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYC119

    Plasmid

    #63916

    Purpose

    Recipient overexpressing vector to construct FLAG tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    FLAG

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. scribble cDNA

    Plasmid

    #42064

    Depositor

    Norbert Perrimon

    Article

    Bilder et al Nature. 2000 Feb 10;403(6770):676-80.

    Insert

    scribble (scrib Fly)
    

    Available Since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

23. AAV-hRedOpsin-TGFB3

    Plasmid

    #164429

    Purpose

    AAV plasmid expressing TGFB3 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

    Insert

    Tgfb3 (Tgfb3 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human red opsin

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAc5.1B-lambdaN-HA-DmGW182

    Plasmid

    #22406

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    gawky, variant A (gw Fly)
    

    Use

    Drosophila expression

    Tags

    lambdaN-HA

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEF-HA-Net1

    Plasmid

    #28264

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Tags

    2x HA

    Expression

    Mammalian

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

26. G273D HA-dynamin 1 pUHD10-3

    Plasmid

    #34689

    Depositor

    Sandra Schmid

    Article

    Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

    Insert

    dynamin 1 (DNM1 Human)
    

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    G273D

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

27. Split-RESCUE-C

    Plasmid

    #170154

    Purpose

    AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    RESCUE-DDC-λN-NES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pJH579

    Plasmid

    #172163

    Purpose

    pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para (pylBCD JM10.1); AraC
    

    Expression

    Bacterial

    Mutation

    Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. SEPT11 sh1 shRNA

    Plasmid

    #122020

    Purpose

    Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 shRNA (Sept11 Rat)
    

    Use

    RNAi

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-KG/Net1

    Plasmid

    #28260

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only