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pcDNA3.1-hACE2-LgBiT

Plasmid

#215837

Purpose

Expresses hACE2 receptor with LgBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

hACE2-LgBiT (ACE2 )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHygG2

Plasmid

#200066

Purpose

hygR selection nls-mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAd5-B7

Plasmid

#122557

Purpose

Block 7 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 30888-35938

Use

Synthetic Biology

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCreV

Plasmid

#140132

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

CCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCreV

Plasmid

#140131

Purpose

can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

Depositor

Hongkui Zeng

Article

Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

Insert

NCreV

Use

AAV

Promoter

EF1a

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSCBE3

Plasmid

#133966

Purpose

Expresses BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

BE3

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PL-452 C-CyPet

Plasmid

#19176

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

CyPet

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFastBacMam2_N_His8_eGFP_TEV

Plasmid

#178806

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins

Depositor

Kazuhiro Abe

Type

Empty backbone

Tags

His8, eGFP, TEV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFRETtv-2in1-CN

Plasmid

#105119

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mTRQ2 and mVenus

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB552

Plasmid

#74882

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB421

Plasmid

#74815

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCWX-R4-DEST-R2-PGR65

Plasmid

#45953

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pRSCupGFP

Plasmid

#1086

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

GFP

Expression

Yeast

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pAd5-B6 ∆E3-GFP

Plasmid

#179201

Purpose

Block 6 with deletion in E3 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6, with deletion in E3 and insertion of GFP expression cassette

Use

Adenoviral

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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p4xEGFP-N1

Plasmid

#122164

Purpose

Expression of 4xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

4xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT EGFP SV40 pA

Plasmid

#69911

Purpose

Expresses EGFP in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

EGFP

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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XW54

Plasmid

#65839

Purpose

Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

NLS-QF-BD-DM-Zipper

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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BRPF1B-c001

Plasmid

#53620

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

BRPF1B

Tags

His-6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only