19,658 results

1. pMVA2RBS035

   Plasmid

   #121051

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

   Plasmid

   #61590

   Purpose

   Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

   Depositor

   David Komander

   Article

   Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

   Insert

   TRABID (ZRANB1 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-244 and aa 698-708.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

   Plasmid

   #31476

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV d14 GFP donor EF1s I-SceI
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   GFP has 14 amino acids deleted from c terminus

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-mTurquoise2-IL-1beta-mNeonGreen

   Plasmid

   #166783

   Purpose

   FRET analysis (pro-IL-1beta cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-IL-1b-mNeongreen (IL1B Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTagBFP2-Lysosomes-20

   Plasmid

   #55308

   Purpose

   Localization: Lysosome Membrane, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   LAMP1
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. opto-RhoA-mCherry_pcDNA3.1

   Plasmid

   #164472

   Purpose

   Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

   Insert

   opto-RhoA
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE-Slow-FT

   Plasmid

   #31915

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Slow-FT
   

   Expression

   Mammalian

   Mutation

   M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInt-ts

    Plasmid

    #66076

    Purpose

    The pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    lambda integrase
    

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-TLR2-YFP

    Plasmid

    #13016

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCAR_Cas9-hygro_GFP

    Plasmid

    #162075

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. LM670

    Plasmid

    #191552

    Purpose

    LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Tags

    HIS-tag

    Expression

    Bacterial

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Flag-YAP-5SA/S94A

    Plasmid

    #33103

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    YAP (YAP1 )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S94A, S109A, S127A, S164A, S381A

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-FOXC1

    Plasmid

    #153114

    Purpose

    Expression of Flag tagged human FOXC1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXC1 (FOXC1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGIG-6xHIS.Mm.RPE65-eGFP

    Plasmid

    #41019

    Depositor

    John Nickerson

    Insert

    RPE65 (Rpe65 Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJG100

    Plasmid

    #138966

    Purpose

    Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Expression

    Worm

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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