We narrowed to 4,504 results for: pcr

1. pFA6a-kanMX6

   Plasmid

   #39296

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-GFP(S65T)-TRP1

   Plasmid

   #41597

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (S65T) and yeast ADH1 terminator

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-His3MX6

   Plasmid

   #41596

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pG-HIV-2LTR

   Plasmid

   #104590

   Purpose

   standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   2LTR circle junction of HIV-1
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5 HA3-eDHFR-T2A-puro

   Plasmid

   #86395

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A puro
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A puro

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMH5

   Plasmid

   #52531

   Purpose

   expresses FLP recombinase in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   FLP recombinase (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   ubiquitin

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pG-HIV-LRT

   Plasmid

   #104592

   Purpose

   Standard for qPCR

   Depositor

   Kristine Yoder

   Article

   Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

   Insert

   HIV-1 late reverse transcripts
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-TRP1-PGAL1-3HA

   Plasmid

   #41609

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, TRP1 reverse orientation, and yeast ADH1 te…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-TRP1-PGAL1-GFP

    Plasmid

    #41615

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP(S65T), TRP1 reverse orientation, and yeast AD…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAH1

    Plasmid

    #41825

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7, TEF

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-TRP1-PGAL1-GST

    Plasmid

    #41612

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GST, TRP1 reverse orientation, and yeast ADH1 ter…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKF251

    Plasmid

    #52532

    Purpose

    Double-GFP reporter for DNA cleavage

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Double-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-13Myc-His3MX6

    Plasmid

    #41602

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    13Myc, A. gossypii translation elongation factor …

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-13Myc-kanMX6

    Plasmid

    #39294

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    13Myc, A. gossypii translation elongation factor …

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-3HA-kanMX6

    Plasmid

    #39295

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    3HA, A. gossypii translation elongation factor 1a…

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. STAGR_gRNAScaffold_hU6

    Plasmid

    #102840

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_hU6promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-GFP(S65T)-kanMX6

    Plasmid

    #39292

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-GST-kanMX6

    Plasmid

    #39293

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFA6a-GST-His3MX6

    Plasmid

    #41604

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-kanMX6-PGAL1

    Plasmid

    #41605

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFA6a-His3MX6-PGAL1

    Plasmid

    #41607

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Tags

    HA and NLS

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. STAGR_gRNAScaffold_h7SK

    Plasmid

    #102842

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_h7SKpromoter
    

    Use

    As pcr template

    Tags

    Expression

    Mutation

    Promoter

    h7SK

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR-SUMO1

    Plasmid

    #114388

    Purpose

    pDONR vector with SUMO1 gene

    Depositor

    Vincent Dion

    Article

    Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q29H compared with NCBI reference NP_003343.1

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHyg-His6

    Plasmid

    #212776

    Purpose

    C-terminal PCR-based tagging with His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMCRW2

    Plasmid

    #127380

    Purpose

    pBC with 4 repeats of 80bp DNA template

    Depositor

    Richard C. Willson

    Article

    Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

    Insert

    4 repeats of 80 bp DNA template sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pG-HIV-SIN-2LTR

    Plasmid

    #104591

    Purpose

    Standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    2LTR circle junction of self-inactivating HIV-based vectors
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBAD-TRP32

    Plasmid

    #71730

    Purpose

    inducible expression of TRP32 in E.coli

    Depositor

    Jere McBride

    Article

    Luo et al Infect Immun. 2008 Apr;76(4):1572-80. do

    Insert

    TRP32
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only