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Showing: 1281 - 1320 of 15188 results

  3. pQadd1F

    Plasmid
    #135660
    Purpose
    Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.
    Depositor
    Andrew Tolonen
    Article
    Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007
    Insert
    Intron containing lox site
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFer
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135660-plasmid-map-sequence-id-305687

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  5. pSTAP-seq_fly-ssp3

    Plasmid
    #86382
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ssp3 enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86382-plasmid-map-sequence-id-173943

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  6. pSTAP-seq_fly-ham

    Plasmid
    #86383
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ham enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86383-plasmid-map-sequence-id-173939

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  7. pSTAP-seq_fly-sgl

    Plasmid
    #86384
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster sgl enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86384-plasmid-map-sequence-id-173940

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  8. pSTAP-seq_fly-ncm

    Plasmid
    #86385
    Purpose
    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster ncm enhancer
    Use
    Stap-seq screening vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86385-plasmid-map-sequence-id-173945

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  32. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  34. PP-ClpR

    Plasmid
    #32876
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ClpR (PF3D7_1436800 Plasmodium Falciparum)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pJ1-g1g2-K2

    Plasmid
    #218217
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatible
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218217-plasmid-map-sequence-id-438339

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  36. pK7-g7g8-K8

    Plasmid
    #218220
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218220-plasmid-map-sequence-id-432226

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  37. pK3-g3g4-K4

    Plasmid
    #218218
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218218-plasmid-map-sequence-id-432228

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  38. pK5-g5g6-K6

    Plasmid
    #218219
    Purpose
    Allows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
    Depositor
    Daniel Voytas
    Insert
    AtU6 promoters and sgRNA scaffolds
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    218219-plasmid-map-sequence-id-432227

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  40. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid
    #115694
    Purpose
    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer
    Article
    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:
    Inserts
    NmeCas9
    Nme-sgRNA
    Use
    CRISPR
    Tags
    NLS and NLS, HA
    Expression
    Insect and Mammalian
    Mutation
    None and fusion of repeat/tracrRNA to make a sgRNA
    Promoter
    EF-1 alpha and U6
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    115694-plasmid-map-sequence-id-221986

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Showing: 1281 - 1320 of 15188 results