We narrowed to 2,735 results for: ubiquitin
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Plasmid#131247PurposeMammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST
Plasmid#208047PurposeEnables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidinDepositorInsertRNF4 (RNF4 Human)
UseLentiviralTagsBirAoptMutationCys>Ala mutations in conserved RING domainPromoterEFSAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B Q116P G120
Plasmid#129289PurposeExpresses 3xFLAG-tagged deconjugation-resistant LC3B in mammalian cellsDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
Tags3xFLAGExpressionMammalianMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD5 (OTU, aa 171-358)
Plasmid#61415PurposeExpresses human OTUD5 (OTU domain) in E. coli.DepositorInsertOTUD5 (OTUD5 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-170 and aa 359-571.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA USP7
Plasmid#131252PurposeMammalian expression of N-terminally Myc-tagged DW164AA USP7DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationContains a point mutation that converts USP7 aspa…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DED/AAA USP7
Plasmid#131253PurposeMammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationContains point mutations that convert USP7 aspart…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCVpuro-6xHis_SUMO2
Plasmid#164938PurposeExpresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNADepositorInsertSmall Ubiquitin Like Modifier 2 (SUMO2 Human)
UseRetroviralTags6XHISPromoterPGK promoterAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)
Plasmid#119315Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cellsDepositorAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236A-pcDNA3.1
Plasmid#85764Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236ADepositorInsertUSPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
TagsHAExpressionMammalianMutationcatalytic inactive mutant C236APromoterCMVAvailable SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
USPL1cat-C236S-pEYFP_C1
Plasmid#85767Purposemammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236SDepositorInsertUSPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
TagsEYFPExpressionMammalianMutationcatalytic domain (aa 212-514), catalytic inactiv…PromoterCMVAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236S-pcDNA3.1
Plasmid#85763Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236SDepositorInsertUSPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
TagsHAExpressionMammalianMutationcatalytic inactive mutant C236SPromoterCMVAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-Flag-Ovtm
Plasmid#51888Purposeexpression vectore in mammalian cellsDepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-Cul4A-deltaROC
Plasmid#20713DepositorInsertCullin 4A (CUL4A Human)
TagsHA2ExpressionMammalianMutationDeletion of residues 594 to 612 from N-terminusAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 N-t
Plasmid#89980PurposeExpresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
TagsGFPExpressionMammalianMutationconstruct contains aa 1-182; deleted aa 183-361Available SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)
Plasmid#119319Purposelentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 C-t
Plasmid#89981PurposeExpresses the GFP-tagged C-terminus of ataxin-3DepositorAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GP805
Plasmid#164545PurposeExpresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717RDepositorInsertSmoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
UseLentiviralTagsFLAGMutationAll 21 cytoplamic Lysines of Smoothened changed t…PromoterGgCryD1Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pfast bac cry1myc
Plasmid#51892PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)
Plasmid#119312Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cellsDepositorAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)
Plasmid#119311Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cellsDepositorAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)
Plasmid#119316Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cellsDepositorAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
Plasmid#85821PurposeiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertZf Ubi::cMyc oDam_f_GFPoNLS
UseTol2 based vectorTagscMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…MutationL122A. Codon optimization compatible to work in M…Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pfast bac Rbx-HA
Plasmid#51893PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_NLS~WT POLH
Plasmid#246405PurposeExpresses WT POLH with an N-terminal NLS~FLAG tag in mammalian cellsDepositorInsertPOLH (POLH Human)
TagsNLS~FLAGExpressionMammalianMutationThe coding sequence for WT POLH has been optimise…Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_ NLS~K682A_K686A_K694_K709A (4KA) POLH
Plasmid#246406PurposeExpresses K682A_K686A_K694_K709A POLH with an N-terminal NLS~FLAG tag in mammalian cellsDepositorInsertPOLH (POLH Human)
TagsFLAGExpressionMammalianMutationPOLH K682A_K686A_K694_K709A. Coding sequence has …Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_ NLS~K682R_K686R_K69R_K709R (4KR) POLH
Plasmid#246407PurposeExpresses K682R_K686R_K69R_K709R POLH with an N-terminal NLS~FLAG tag in mammalian cellsDepositorInsertPOLH (POLH Human)
TagsFLAGExpressionMammalianMutationPOLH K682R_K686R_K69R_K709R. Coding sequence has …Available SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLH_ΔGG NEDD8
Plasmid#221860PurposeExpresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cellsDepositorInsertPOLH (POLH Human)
TagsFLAG and ΔGG NEDD8ExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_K682A_K709A POLH
Plasmid#221864PurposeExpresses POLH mutated at K682A and K709A with a FLAG tag in mammalian cellsDepositorInsertPOLH (POLH Human)
TagsFLAGExpressionMammalianMutationPOLH K682A + K709A. Coding sequence has been opti…PromoterCMVAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_K682A_K686A_K694A_K709A POLH
Plasmid#221865PurposeExpresses POLH mutated at K682A, K686A, K694A and K709A with a FLAG tag in mammalian cellsDepositorInsertPOLH (POLH Human)
TagsFLAGExpressionMammalianMutationPOLH K682A, K686A, K694A + K709A. Coding sequence…PromoterCMVAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMAPLe4 PCMTD1 PCMTD1(∆ESB-ASB9)
Plasmid#246507PurposeRecombinant protein expression of PCMTD1(∆ESB-ASB9)DepositorInsertProtein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
TagsHis-tagExpressionBacterialMutationDeleted amino acids 266-340 in PCMTD1 and replace…Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+) PCMT1 H58 A214
Plasmid#246496PurposeRecombinant protein expression of PCMT1 H58 A214DepositorInsertProtein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)
TagsHis-tagExpressionBacterialMutationThreonine 58 mutated to Histidine and Valine 214 …PromoterT7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_ΔC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ΔC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_FL
Plasmid#187722PurposeExpress His-tagged NEDD4L FL in insect cellsDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_FL
Plasmid#187705PurposeExpress his-tagged NEDD4L FL in bacterial cellsDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W4-HECT
Plasmid#187710PurposeExpress his-tagged NEDD4L W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 543-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-542Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_WW34
Plasmid#187718PurposeExpress GST-tagged NEDD4L WW34 tandem domains in bacterial cellsDepositorInsertNEDD4L residues 492-581 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationonly residues 492-581Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmch-mTet1-ZF-CD pc3956
Plasmid#201709PurposeExpresses of mcherry-tagged mTet1-ZF-CD in mammalian cellsDepositorInsertTet1-ZF_CD (Tet1 Mouse)
TagsmCherryExpressionMammalianMutationZinc finger domain of Tet1 fused to its catalytic…PromoterCAGAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA 1-205 USP7
Plasmid#131254PurposeMammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tagDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationExpresses a mutant form of our pcDNA3.1-N-Myc_1-2…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B Q116P G120
Plasmid#129290PurposeExpresses GFP-tagged deconjugation-resistant LC3B in mammalian cellsDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsGFPExpressionMammalianMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterCMVAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [dRING]
Plasmid#126549PurposeExpresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationRING domain deletion mutant [dRING mutation is a …PromoterLTRAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)
Plasmid#119318Purposelentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (G20V)
Plasmid#119308Purposelentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (C21Y)
Plasmid#119304Purposelentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)
Plasmid#119313Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)
Plasmid#119317Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cellsDepositorAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)
Plasmid#119310Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cellsDepositorAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only