We narrowed to 63,599 results for: cell

1. Human NANOS3-P2A-mCherry knock-in targeting construct

   Plasmid

   #59721

   Purpose

   Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   P2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-EGFP-mAtg5

   Plasmid

   #38196

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTet-O-OVOL1-T2A-PuroR

   Plasmid

   #162347

   Purpose

   Lentiviral expression of OVOL1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   OVOL1 (OVOL1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-neo largeTcDNA K1

   Plasmid

   #8581

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   E107K. Alteration in pRB consensus binding motif…

   Promoter

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pHRSin-IRES2-EmGFP_HPC-4 Ab light

   Plasmid

   #187359

   Purpose

   Lentiviral expression of HPC-4 antibody light chain

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   HPC-4 antibody light chain
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Nrxn1beta AS4(-)-Fc

   Plasmid

   #59313

   Purpose

   Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Peter Scheiffele, Tito Serafini

   Article

   Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

   Insert

   Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
   

   Use

   Tags

   Fc (from human IgG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT_TO_eGFP_CELF1

   Plasmid

   #106100

   Purpose

   Inducible gene expression of EGFP - CELF1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   CELF1 (CELF1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

   Plasmid

   #62957

   Purpose

   Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4 in three phases
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR LAT-FFF/Zap70 iLID-Drop

    Plasmid

    #171032

    Purpose

    Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

    Insert

    LAT (LAT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDendra2-H3

    Plasmid

    #157814

    Purpose

    Fluorescent reporter for histone H3 tracking

    Depositor

    Xin Chen

    Article

    Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

    Insert

    H3 (H3c1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PH Akt-Venus

    Plasmid

    #85223

    Purpose

    Venus tagged PH domain of Akt for use as a PIP3 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    PH AKT(1-148) (AKT1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro H-Ras G12V T35S

    Plasmid

    #12274

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V T35S (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The T35S mutat…

    Promoter

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid

    #59314

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

    Depositor

    Peter Scheiffele, Tito Serafini

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACT2-μ3A

    Plasmid

    #198174

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitution in codon 225

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. DR.GFP

    Plasmid

    #17617

    Depositor

    Linzhao Cheng

    Article

    Cui et al Blood. 2002 Jan 15. 99(2):399-408.

    Insert

    HLA-DRa promoter (HLA-DRA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. ELF3_pet28a

    Plasmid

    #131658

    Purpose

    Bacterial expression of ETS gene ELF3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF3 (ELF3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ EB3-mKate2

    Plasmid

    #105940

    Purpose

    in vitro transcription

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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