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We narrowed to 30,495 results for: des.2

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Showing: 6661 - 6680 of 30495 results

  1. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid
    #236174
    Purpose
    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi
    Inserts
    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    CMV and four ZNF35/IKZF1 sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236174-plasmid-map-sequence-id-468370

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  3. Venus-Puma-4E-pEGFP-C1

    Plasmid
    #166746
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Puma-4E (BBC3 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166746-plasmid-map-sequence-id-329007

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  5. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  6. His-ZZ-TEV-SNAPf-DYNC2H1

    Plasmid
    #132524
    Purpose
    Full length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragments
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Insert
    DYNC2H1 (DYNC2H1 Human)
    Tags
    8x His, Linker-TEV-linker-TEV, Precission cleavag…
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    132524-plasmid-map-sequence-id-261476

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  8. pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1

    Plasmid
    #229510
    Purpose
    Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1
    Depositor
    Laura Gunn
    Article
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
    Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
    RbcX2 (RbcX2 Mustard Weed)
    BSD2
    RbcX1
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available Since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229510-plasmid-map-sequence-id-460710

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  13. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  14. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  17. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid
    #236230
    Purpose
    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    Use
    AAV
    Tags
    mEmerald
    Promoter
    human Synapsin 1
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236230-plasmid-map-sequence-id-471247

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  19. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid
    #236245
    Purpose
    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter
    Depositor
    Jennifer Lippincott-Schwartz
    Article
    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do
    Insert
    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    Use
    AAV
    Tags
    mTagBFP2
    Promoter
    U6 and human Synapsin1
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    236245-plasmid-map-sequence-id-471239

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Showing: 6661 - 6680 of 30495 results