163,777 results

1. GFP-GCN5

   Plasmid

   #65386

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   GCN5 (KAT2A Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Wnt3

   Plasmid

   #35909

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt3 (WNT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.22-VEGF-HRE::dLUC

   Plasmid

   #128096

   Purpose

   Hypoxia response element real-time luciferase reporter

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
   

   Use

   Luciferase

   Tags

   drives expression of destabilized luciferase (2CP…

   Expression

   Mammalian

   Mutation

   Promoter

   inserted VEGF hypoxia response elements (HRE) (x5)

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-O-FUW-Brn2

   Plasmid

   #27151

   Depositor

   Marius Wernig

   Article

   Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV Puro SCRIB WT

   Plasmid

   #88886

   Purpose

   SCRIB WT in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Tags

   N/A

   Expression

   Mutation

   N/A

   Promoter

   MSCV promoter

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 CIM5 (Rpt-1) GFP

   Plasmid

   #16074

   Depositor

   Erin Schuman

   Article

   Bingol et al Nature. 2006 Jun 29. 441(7097):1144-8.

   Insert

   CIM5 (RPT1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166016

   Purpose

   mammalian expression of HCoV-229E soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   HCoV-229E S (spike) (S )
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-NLS-actin

   Plasmid

   #118380

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   NLS

   Promoter

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUGW-H1-Scrambled control shRNA

   Plasmid

   #40625

   Depositor

   Sally Temple

   Article

   Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

   Insert

   Scrambled control shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. yTREX

    Plasmid

    #177306

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

    Depositor

    Karl-Erich Jaeger

    Article

    Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti6MFV SLC40A1

    Plasmid

    #194132

    Purpose

    Expresses MTP1 (SLC40A1) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC40A1 (SLC40A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVS1-VIR2

    Plasmid

    #134745

    Purpose

    Virulence and replication helper

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    vir gene cluster
    

    Use

    Vir gene expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM444

    Plasmid

    #108319

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA

    Plasmid

    #171619

    Purpose

    AAV floxed Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    IE94-Flex-TM-KA2-Cam-NES-TeVN-AsLOV2-TEVseq-bGH-PA
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4134902

    Plasmid

    #151912

    Purpose

    Mammalian expression plasmid for Human coronavirus 229E nucleocapsid

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    Human coronavirus 229E nucleocapsid protein (N Human coronavirus 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET42b-AncBE4max

    Plasmid

    #165157

    Purpose

    Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    AncBE4max
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTRIP-CMV-GFP-FLAG-cGAS

    Plasmid

    #86675

    Purpose

    Expresses catalytically-active GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-human Erbb2-IRES-GFP

    Plasmid

    #91888

    Purpose

    Retroviral expression of human Erbb2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Erbb2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV gag

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. tetO7_Ef1a_SOX2

    Plasmid

    #18919

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

21. TurboID-EV

    Plasmid

    #215085

    Purpose

    Expresses TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228360

    Purpose

    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pME-GAL4-ERT2-VP16

    Plasmid

    #82588

    Purpose

    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    GAL4-ERT2-VP16
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GPR27-Tango

    Plasmid

    #66349

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR27 (GPR27 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3 - hSRSF6

    Plasmid

    #120419

    Purpose

    overexpression of human SRSF6

    Depositor

    Gillian Bates, Andreas Neueder

    Article

    Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

    Insert

    SRSF6 (SRSF6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate/early

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1 ZoAl-ENT N-1F (R1103A)

    Plasmid

    #216548

    Purpose

    For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOD2045-intControl

    Plasmid

    #100005

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-NLS-HA-TurboID-PCNA

    Plasmid

    #215076

    Purpose

    Expresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    NLS-HA-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHes1-DsRed

    Plasmid

    #13767

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Hes1 promoter
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge