161,600 results

1. Cas9 TLS1

   Plasmid

   #196977

   Purpose

   Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS1 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21a-GluRS-His

   Plasmid

   #124109

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Glutamyl-tRNA ligase (gltX Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-UCOE-EF1a-Zim3-dCas9-BFP

   Plasmid

   #188775

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

   Plasmid

   #162378

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8m
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   Synapsin

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CAG FLEX BARK p2A mCherry

   Plasmid

   #117693

   Purpose

   CAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs peptide
   

   Use

   Cre/Lox

   Tags

   HA / FLAG

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUBC-Pfv-Sapphire_PURO

   Plasmid

   #203651

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1P shSOD2

   Plasmid

   #102976

   Purpose

   Suppression of SOD2

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shSOD2 (SOD2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBFP2-C1

   Plasmid

   #22880

   Depositor

   Dorus Gadella

   Article

   Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

   Insert

   SBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pT7-NSP4SA11

   Plasmid

   #89171

   Purpose

   Express Rotavirus SA11 NSP4 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 NSP4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AICSDP-19:LAMP1-mEGFP

    Plasmid

    #101782

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

    Depositor

    Allen Institute for Cell Science

    Insert

    LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLL3.7

    Plasmid

    #11795

    Purpose

    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

    Depositor

    Luk Parijs

    Article

    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHel2

    Plasmid

    #102960

    Purpose

    E.coli/H.Pylori shuttle vector

    Depositor

    Rainer Haas

    Article

    Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

    Type

    Empty backbone

    Use

    Plasmid shuttle vector system for genetic complem…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMAL-c5x_UnaG

    Plasmid

    #163125

    Purpose

    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    UnaG
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. V5-APEX2-SENP2

    Plasmid

    #129276

    Purpose

    Expression of APEX2 at nuclear pore

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    V5-APEX2-SENP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMYH9-D1424H-FLAG

    Plasmid

    #183513

    Purpose

    Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Insert

    MYH9 (MYH9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Aspartic acid 1424 to Histidine

    Promoter

    CMV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GalL

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eGFPC1 FKBP

    Plasmid

    #67529

    Purpose

    Expresses GFP fused FKBP

    Depositor

    Stephen Tait

    Article

    Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

    Insert

    FKBP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    F36V

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VPS29-YFP

    Plasmid

    #174520

    Purpose

    Expresses human VPS29 with a C-terminal YFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Rojas et al J Cell Biol. 2008 Nov 3;183(3):513-26. d

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF5B-FRT-GFP-αTAT1

    Plasmid

    #27099

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

    Insert

    α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
    

    Use

    Tags

    GFP, S-tag, and TEV site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE2 Lnp-mCherry

    Plasmid

    #86687

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    LNPK (LNPK Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Miguel Angel Moreno-Mateos, Julián Cerón

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SIVh

    Plasmid

    #81091

    Purpose

    Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HBx

    Plasmid

    #65463

    Purpose

    expresses HBx in mammalian cells

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al Hepatology. 2004 Jun;39(6):1683-93.

    Insert

    HBV X protein
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. spisPink chromoprotein

    Plasmid

    #117839

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, spisPink
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-puro GFP-p62

    Plasmid

    #38277

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTAT-HA

    Plasmid

    #35612

    Depositor

    Steven Dowdy

    Article

    Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

    Type

    Empty backbone

    Use

    Tags

    6xHIS, HA, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

    Plasmid

    #218860

    Purpose

    10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCG-ICAM(1235)-nrxn1b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDE43-MCSq17

    Plasmid

    #27333

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    qtag17
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-Foxo1 (pCMV5)

    Plasmid

    #12142

    Depositor

    Domenico Accili

    Article

    Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

    Insert

    Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Wild-type

    Promoter

    CMV

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge