We narrowed to 11,723 results for: Vars

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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TERT - R865C

Plasmid

#213926

Purpose

Expresses Mutant TERT R865C

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Mutant TERT R865C (TERT Human)

Use

Lentiviral

Tags

6x His Tag

Expression

Mammalian

Mutation

Arginine 865 changed to Cysteine

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CSF1R-V5/HIS

Plasmid

#201984

Purpose

expression of human CSF1R receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

CSF1R receptor tyrosine kinase (CSF1R Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_WT

Plasmid

#82216

Purpose

Gateway Donor vector containing SMAD4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FOXL2

Plasmid

#192914

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 (FOXL2 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUDP082

Plasmid

#103875

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBL1XR1_WT

Plasmid

#82059

Purpose

Gateway Donor vector containing TBL1XR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBL1XR1 (TBL1XR1 Human)

Use

Gateway entry vector

Mutation

L512P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT6_WT

Plasmid

#81950

Purpose

Gateway Donor vector containing STAT6, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT6 (STAT6 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Article

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Hsp68

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHPSE-P6

Plasmid

#185907

Purpose

Engineered Human Heparanase mutant for e.coli expression

Depositor

Colin Jackson

Article

Whitefield et al RSC Chem Biol. 2022 Feb 15;3(3):341-349.

Inserts

HPSE1 (HPSE Human)
HPSE1 (HPSE Human, Synthetic)

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUDP025

Plasmid

#103874

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-Q146H-FLAG-IRES-GFP

Plasmid

#69048

Purpose

lentiviral expression of IKZF1 variant 1 with Q146H mutation and FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

splice variant 1, Q146H

Promoter

CAG

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4E

Plasmid

#224452

Purpose

Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDFNNT insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV1A

Plasmid

#224437

Purpose

Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDLTTP insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

Plasmid

#228253

Purpose

pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

mpknot entry plasmid backbone for epegRNA expression via human U6 promoter

Expression

Mammalian

Mutation

n/a

Promoter

U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN-SRF

Plasmid

#232629

Purpose

Lentiviral plasmid expressing human SRF.

Depositor

Claes Wadelius

Article

Christensen et al Eur Heart J. 2025 Apr 10:ehaf213. doi: 1

Insert

SRF (SRF Human)

Use

Lentiviral

Tags

HA-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF2_WT

Plasmid

#82239

Purpose

Gateway Donor vector containing TRAF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF2 (TRAF2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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