We narrowed to 17,772 results for: STO

1. pT2K-p53DN-T2A-copGFP

   Plasmid

   #109229

   Purpose

   For Tol2 transposon-mediated stable expression of dominant negative p53 and copGFP

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   dominant negative p53 (Trp53 Mouse)
   

   Tags

   copGFP

   Expression

   Mammalian

   Mutation

   dominant negative p53

   Promoter

   CAGGS

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5_mEGFP-TAZ-CAMTA1-KS

   Plasmid

   #237677

   Purpose

   For overexpression of mEGFP-TAZ-CAMTA1-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   TAZ-CAMTA1-KS (WWTR1, CAMTA1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-7xTRE-3xeGFP

   Plasmid

   #191206

   Purpose

   GFP expression under the control of TRE promotor

   Depositor

   Janelia Viral Tools

   Article

   Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

   Insert

   3X GFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

   Plasmid

   #159698

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-2dV-Camui (T305D/T306D)

   Plasmid

   #220368

   Purpose

   T305D/T306D phosphomimetic mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T305D/T306D)-mEGFP(A206K) (Camk2a Synthetic, Rat)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed both Threonine 305 and 306 to aspartic ac…

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-mTubb3

   Plasmid

   #87116

   Purpose

   AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
   

   Use

   AAV and CRISPR

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIH-mRuby2-BAK

   Plasmid

   #111627

   Purpose

   Fluorescent fusion protein used to visualise mouse BAK, with hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   BAK (Bak1 Mouse)
   

   Use

   Retroviral

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

   Plasmid

   #159680

   Purpose

   Mammalian protein expression of KRAS4a-Halotag fusion

   Depositor

   Dominic Esposito

   Article

   Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

   Insert

   Halotag-Hs.KRAS4a (KRAS Human)
   

   Tags

   Halotag

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ezh2[SET]-dCas9

   Plasmid

   #100087

   Purpose

   Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   Ezh2[SET] (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[SET]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Ezh2[SET] includes aa 482-746

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-1BPNLS-Cas9-1BPNLS

    Plasmid

    #87108

    Purpose

    BPNLS-Cas9-BPNLS expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-KIF1B beta-600-1400

    Plasmid

    #79109

    Purpose

    Mammalian expression of GFP-KIF1B beta-600-1400

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pMx-MGT

    Plasmid

    #111810

    Purpose

    Retroviral expression vector for direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

    Insert

    Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    viral LTR

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid

    #153062

    Purpose

    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Mutagenesis to insert a stop codon at aa 298 in B…

    Promoter

    EF1α

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 NL-BCL2L1

    Plasmid

    #113458

    Purpose

    NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    BCL2 like 1 (BCL2L1 Human)
    

    Use

    Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZ:F3-CAGGS GPHTK-F

    Plasmid

    #112666

    Purpose

    Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    eGFP
    

    Use

    Gene targeting vector for integration at the s1 s…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpp1-is2

    Plasmid

    #58248

    Purpose

    Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Spp1-is2 (Spp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

    Plasmid

    #181850

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. R725-X11-566: His6-MBP-tev-Hs.LGALS3

    Plasmid

    #159699

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.LGALS3

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112668

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRSFDuet-FBXL5-SKP1

    Plasmid

    #226620

    Purpose

    Co-expression of FBXL5-SKP1 complex in E.coli

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Inserts

    FBXL5 (FBXL5 Human)
    SKP1 (SKP1 Human)
    

    Tags

    GB1

    Expression

    Bacterial

    Mutation

    Amino acids 1-198 was deleted, and 420-596 was re…

    Promoter

    T7

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT2K-LRL-mouse c-MYC-IRES-Luc2

    Plasmid

    #109228

    Purpose

    For Tol2 transposon-mediated stable expression of LRL, c-Myc and IRES-luciferase

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

    Insert

    c-Myc (Myc Mouse)
    

    Tags

    IRES-Luc2

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)

    Plasmid

    #85800

    Purpose

    donorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2Bneon

    Depositor

    Roland Friedel

    Article

    Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

    Insert

    PPP1R12C (PPP1R12C Human)
    

    Use

    Human targeting

    Mutation

    homology arms for knock-in into first intron

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSpp1-is3

    Plasmid

    #58247

    Purpose

    Expression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    Spp1 isoform 3 (Spp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLentiCRISPRv1-sgAAVS1

    Plasmid

    #83906

    Purpose

    stable knockout

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    adeno-associated virus integration site 1 (AAVS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

    Plasmid

    #209060

    Purpose

    Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

    Depositor

    Matthew Oser

    Article

    Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

    Insert

    sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
    

    Use

    Gateway vector to be used for lr reaction

    Promoter

    U6

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

    Plasmid

    #162618

    Purpose

    Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

    Depositor

    Marco Morsch

    Article

    Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

    Insert

    TARDBP (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    M337V

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET30-2-PHGDH-C234S

    Plasmid

    #107724

    Purpose

    Expression of PHGDH C234S in bacteria

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    PHGDH (PHGDH Human)
    

    Expression

    Bacterial

    Mutation

    C234S

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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