59,544 results

1. pT7-V5-SBP-C1-HsGIGYF1_AI

   Plasmid

   #148961

   Purpose

   Mammalian Expression of HsGIGYF1

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

   Insert

   HsGIGYF1 (GIGYF1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pE-N1-RDH11

   Plasmid

   #161916

   Purpose

   Expresses untagged human RDH11. Confers G418 resistance.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   Retinol dehydrogenase 11 (RDH11 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pM201

   Plasmid

   #173179

   Purpose

   dCas9-24xGCN4

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   dCas9-24xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFAM-SNAPf

   Plasmid

   #187909

   Purpose

   Mammalian cell expression of SNAPf-labeled TFAM

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   TFAM (TFAM Human)
   

   Tags

   SNAPf

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV6-AN-DDK_WT RAD23A

   Plasmid

   #201438

   Purpose

   Expresses human RAD23A with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221-CLN3_STOP

   Plasmid

   #161034

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   CLN3 (CLN3 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pMCSF-R-luc

   Plasmid

   #12420

   Depositor

   Dong-Er Zhang

   Article

   Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

   Insert

   M-CSF receptor promoter fragment (CSF1R Human)
   

   Use

   Luciferase

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pHAGE-PDGFRA

   Plasmid

   #116769

   Purpose

   Lentiviral expression of PDGFRA

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAP3K15-TAP

   Plasmid

   #69728

   Purpose

   Express tandem tagged MAP3K15 in cells

   Depositor

   Daniel Liebler

   Insert

   Apoptosis signal-regulating kinase 3 (MAP3K15 Human)
   

   Tags

   HA-Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. alpha Synuclein-VC

    Plasmid

    #89471

    Purpose

    expresses human alpha Synuclein fused to the C-terminal part of Venus

    Depositor

    Bradley Hyman, Tiago Outeiro

    Article

    Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

    Insert

    SNCA (SNCA Human)
    

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223-SRC

    Plasmid

    #23934

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SRC (SRC Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. PCMV4-3 HA/IkB-alpha(SS32,36AA)

    Plasmid

    #24143

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    IkB alpha (NFKBIA Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    S32A S36A

    Available Since

    March 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQTEV-PSMD11

    Plasmid

    #31333

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223-RAF1

    Plasmid

    #23832

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 HA-PHLPP1 full length

    Plasmid

    #37100

    Purpose

    Expresses HA tagged, full-length human PHLPP1 in mammalian cells

    Depositor

    Alexandra Newton

    Article

    Warfel et al J Biol Chem. 2011 Jun 3;286(22):19777-88

    Insert

    PHLPP1 (PHLPP1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe puro-eIF4E

    Plasmid

    #33252

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    eIF4E (EIF4E Human)
    

    Use

    Retroviral

    Tags

    untagged

    Expression

    Mammalian

    Mutation

    unmodified

    Promoter

    SV40

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pGEX6P1‐hsRNASEH2BCA

    Plasmid

    #108692

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

    Depositor

    Andrew Jackson, Martin Reijns

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon a…

    Promoter

    tac

    Available Since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CYP51A1 (3I3K)

    Plasmid

    #25161

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP51A1_cw_09 (CYP51A1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acid residues 54-502

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PIK3CA - Exon9 E545K

    Plasmid

    #16642

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E545K; see note below

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge