161,273 results

1. pLENTI-CMV-GFP-TREX1 D18N BLAST

   Plasmid

   #164225

   Purpose

   lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D18N

   Promoter

   CMV

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. plk1 T210D

   Plasmid

   #132964

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T210D

   Promoter

   Available since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GW1CMV-Perceval

   Plasmid

   #21737

   Purpose

   can be used to monitor the ATP:ADP ratio during live-cell imaging

   Depositor

   Gary Yellen

   Article

   Berg et al Nat Methods. 2009 Feb . 6(2):161-6.

   Insert

   Perceval (fluorescent ATP:ADP sensor)
   

   Use

   Tags

   5'UTR

   Expression

   Mammalian

   Mutation

   improved Kozak sequence

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

   Plasmid

   #130816

   Purpose

   Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   bJun-NFAST-NLS-DEVDG-mCherry-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEFmEGFP-mDia2

   Plasmid

   #25407

   Depositor

   Arthur Alberts

   Article

   Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

   Insert

   mDia2 (Diaph3 Mouse)
   

   Use

   Tags

   EGFP, His, and Myc

   Expression

   Mammalian

   Mutation

   Encodes mDia2

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA PTEN

   Plasmid

   #78776

   Purpose

   To overexpress PTEN in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEN6-Ubc5 Sc

   Plasmid

   #83234

   Purpose

   pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Inserts

   Ubiqutin (UBC Human)
   Ubc 5 (UBC5 Budding Yeast)
   Uba 1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2plus-mKate2

   Plasmid

   #162624

   Purpose

   Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_ALV_Env

   Plasmid

   #207325

   Purpose

   Expression of env polyprotein from Avian leukosis virus (ALV)

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   ALV_Env (env Avian leukosis virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p104_gRNA_Sp_CD3e

    Plasmid

    #213780

    Purpose

    Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sp gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-Galectin1-9

    Plasmid

    #56434

    Purpose

    Localization: Glycoproteins, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    Galectin1 (LGALS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pcDNA4/TO-HRASG12V

    Plasmid

    #216509

    Purpose

    Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    HRAS (HRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10878

    Plasmid

    #183964

    Purpose

    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK-FLAG-UbxD8

    Plasmid

    #53777

    Purpose

    express FLAG-tagged human UbxD8 gene

    Depositor

    Yihong Ye

    Article

    Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

    Insert

    UbxD8 (FAF2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET-26b-NbVHH05-HA-His

    Plasmid

    #171567

    Purpose

    Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-HA-His (UBE2J1 Human)
    

    Use

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. shYAP1 # 1

    Plasmid

    #42540

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

    Plasmid

    #199349

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLZts

    Plasmid

    #128799

    Purpose

    Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

    Depositor

    Timothy Barnett

    Article

    Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

    Type

    Empty backbone

    Use

    Bacterial mutagenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Rep2

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. NGFR P210

    Plasmid

    #27486

    Depositor

    Warren Pear

    Article

    He et al Blood. 2002 Apr 15;99(8):2957-68.

    Insert

    bcr/abl (P210) (ABL1, BCR Human)
    

    Use

    Retroviral

    Tags

    IRES and NFGR

    Expression

    Mammalian

    Mutation

    Contains the "complete" bcr/abl fusion

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pαH-rS2d-HexaPro

    Plasmid

    #183515

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3/HA-RBP2

    Plasmid

    #14799

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LT1_36_QUEEN-2m

    Plasmid

    #177708

    Purpose

    Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEF1-QUEEN2m
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-Tag2B EDD

    Plasmid

    #37188

    Depositor

    Charles Watts, Darren Saunders

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EMTB-3XGFP

    Plasmid

    #26741

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    3x EGFP and Myc

    Expression

    Mammalian

    Mutation

    Only contains the microtubule binding-domain of e…

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMSCVhyg-H3.3K4M-FLAG/HA

    Plasmid

    #128741

    Purpose

    Expression of histone H3.3K4M-FLAG/HA

    Depositor

    Kai Ge

    Article

    Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

    Insert

    H3.3K4M (H3-3A Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mutation

    K4M

    Promoter

    MSCV

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU6-sgCXCR4-2

    Plasmid

    #46917

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCXCR4 -2 (CXCR4 Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge