171,789 results

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pLX-EF1a_GFP_CdTag-HA

Plasmid

#221492

Purpose

GFP lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ETV6

Plasmid

#131594

Purpose

doxycycline-inducible expression of human ETV6 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

ETV6 (ETV6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX iLOV

Plasmid

#26587

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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AKT3-NanoLuc Fusion Vector

Plasmid

#237208

Purpose

Express AKT3-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

AKT3 (AKT3 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Expression

Bacterial

Mutation

C terminal domain only

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
FSW CHIP-Myc

Plasmid

#246724

Purpose

Neuron-specific expression of human CHIP wildtype, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

Synapsin

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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mEmerald-myo1e

Plasmid

#249264

Purpose

Fluorescently tagged myosin 1e

Depositor

Mira Krendel

Article

Barger et al Nat Commun. 2019 Mar 19;10(1):1249. doi:

Insert

Myo1e (MYO1E Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pZB246-T7-T+

Plasmid

#250903

Purpose

Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone

Depositor

Timothy Whitehead

Article

Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.

Insert

Thermally Stable T7 RNA Polymerase

Tags

6xHis

Expression

Bacterial

Mutation

Arginine 31 to Aspartic Acid, Alanine 61 to Argin…

Promoter

pT5-lacUV

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-epsilon-14-3-3

Plasmid

#250063

Purpose

Bacterial expression of epsilon 14-3-3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Epsilon (YWHAE Human)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a myc tagged mbIL2 IRES miRFP720-P2A-Blast

Plasmid

#248031

Purpose

Constitutively expresses myc tagged membrane bound IL2, miRFP720 and a blasticidin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

myc-tagged membrane bound IL2 (IL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCEFL_GPR161-HA

Plasmid

#249268

Purpose

Expression of GPR161 wild type

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

GPR161 (GPR161 Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pdCas12f1Cas_ABE-LVA

Plasmid

#220995

Purpose

Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-ABE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pVT-Ruby

Plasmid

#234918

Purpose

This all-in-one vector is used to express the Ruby gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Ruby

Expression

Plant

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP VPS39

Plasmid

#245031

Purpose

Protein localization of human wild-type VPS39 in cells

Depositor

Thomas Martin

Insert

VPS39 (VPS39 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCSCG-TM(DPP4)-Fc-hST3Gal4 WT

Plasmid

#239825

Purpose

Displays Fc-fusion hST3Gal4 WT on mammalian cell surface via transmembrane domain of DPP4

Depositor

Sriram Neelamegham

Article

Hombu et al Small. 2025 May 26:e2502318. doi: 10.100

Insert

TM(DPP4)-Fc-hST3Gal4 WT

Use

Lentiviral

Tags

human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-42 of hST3Gal4 were deleted

Promoter

CMV promoter

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_003_Amp/ColEI_mScarlet-I

Plasmid

#235969

Purpose

Ampicillin/ColEI backbone with mScarlet-I placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph0

Plasmid

#231897

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph1

Plasmid

#231898

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-FlpTag-GFP-ph2

Plasmid

#231899

Purpose

Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

SD-GFP-SA

Expression

Bacterial

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph2

Plasmid

#231902

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph1

Plasmid

#231901

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-FlpTag-DsRed-HDR-ph0

Plasmid

#231900

Purpose

dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

Depositor

Alexander Borst

Article

Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

Insert

Frt-Frt14-SD-GFP-SA-Frt-Frt14

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTetON-rHUH

Plasmid

#235192

Purpose

To express rHUH protein in mammalian cells

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

rHUH

Expression

Mammalian

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAG Retro EGFP Neo

Plasmid

#234464

Purpose

Control retroviral vector expressing Green Fluorescent Protein. Neomycin selection.

Depositor

Melina Fan, Addgene Research Program

Insert

Green Fluorescent Protein

Use

Retroviral

Available Since

March 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD-WzxE-TEV-His10

Plasmid

#226781

Purpose

Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

Depositor

James Naismith

Article

Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

Insert

wzxE

Tags

TEV cleavable 10xHistidine tag

Expression

Bacterial

Mutation

valine insertion in position 2 due to the cloning…

Promoter

araBAD

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-MfnG

Plasmid

#191115

Purpose

Lentiviral vector expressing MfnG

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

MfnG

Use

Lentiviral

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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