163,740 results

1. ins:MCS2; cryaa:RFP

   Plasmid

   #110286

   Purpose

   A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Type

   Empty backbone

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   insulin

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTGMP

   Plasmid

   #32716

   Depositor

   Scott Lowe

   Article

   Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pfli1

   Plasmid

   #22413

   Depositor

   Nathan Lawson

   Article

   Lawson et al Development. 2001 Oct . 128(19):3675-83.

   Insert

   fli1 (fli1 Zebrafish)
   

   Use

   Riboprobe

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pLX304-Flag-APEX2-NES

   Plasmid

   #92158

   Purpose

   Cytosolic APEX2 construct

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   Flag-APEX2-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Actin-cpstFRET-Actin

   Plasmid

   #80643

   Purpose

   cpstFRET actin sensor for mechanical force measurements in cytoskeleton

   Depositor

   Fred Sachs

   Article

   Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

   Insert

   Beta actin, cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTarget

   Plasmid

   #214052

   Purpose

   Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Target sequence
   

   Use

   Target rna plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNon-target

   Plasmid

   #214053

   Purpose

   Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Non-target sequence
   

   Use

   Non-target rna plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHochTypeIII

   Plasmid

   #214039

   Purpose

   Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Cas10
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT

   Promoter

   T7 promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+FLAG-KrascomG12D

   Plasmid

   #206844

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available Since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB533_15F11-sfGFP

    Plasmid

    #167526

    Purpose

    H4K20me1-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

    Insert

    15F11-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTBS 5Y YIC

    Plasmid

    #18786

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    TRAP binding site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. 51_pAAV-ProC12-CatCh-GFP-WPRE

    Plasmid

    #125947

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC12

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. At2S3:RUBY

    Plasmid

    #160906

    Purpose

    RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes

    Depositor

    Yunde Zhao

    Article

    He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

    Insert

    RUBY and At2S3 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    At2S3

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-HNRNPA1C-mCh-Cry2WT

    Plasmid

    #101226

    Purpose

    hnRNPA1(186-320) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    hnRNPA1(186-320) (HNRNPA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. 39 p ephrin B2

    Plasmid

    #13063

    Depositor

    Michael Tsang, Nathan Lawson

    Article

    Lawson et al Development. 2001 Oct . 128(19):3675-83.

    Insert

    ephrin B2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 23, 2010

    Availability

    Academic Institutions and Nonprofits only

17. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EGFP-C1-centrin-1

    Plasmid

    #73331

    Purpose

    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

    Depositor

    Manuel Thery

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128346

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Rbfox3 (NeuN) (Rbfox3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-IRES-GFP-mouse-Caspase-11

    Plasmid

    #183358

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-11 (Casp4 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid

    #105802

    Purpose

    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-mRuby3; ORF2: TEV-mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CMV-dSa-VPR mini.-1X snRP1

    Plasmid

    #99687

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. LentiV2-hU6-evopreQ1

    Plasmid

    #210189

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMC_mClover3_BSDminus_F0

    Plasmid

    #184165

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV HA hRB-wt

    Plasmid

    #58905

    Purpose

    mammalian expression of wild type human Rb

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

    Plasmid

    #229787

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; ZF10-NS3-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLminP_Luc2P_RE16

    Plasmid

    #90357

    Purpose

    CREB - Cyclic AMP gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CREB

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDH_CMV_TFAM_mKate2

    Plasmid

    #167331

    Purpose

    Expresses mKate2 fused to full length TFAM in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge