We narrowed to 1,677 results for: tyr
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Plasmid#123209PurposeGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal taggingDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneMutationStop codon removedAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAPL1 G116A
Plasmid#123084PurposeGateway entry clone encoding human GABARAPL1 G116ADepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseGateway entry vector / entry cloneMutationGlycine 116 to AlanineAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP G116A
Plasmid#123098PurposeExpresses 3xFLAG-GABARAP G116A in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
Tags3xFLAGExpressionMammalianMutationGlycine 116 to AlaninePromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Ptgs2_Balbc
Plasmid#170301PurposeA knock-out vector for the mouse Ptgs2 in Balb/cDepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RCK-Y1699C
Plasmid#25067DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains only the ROC-COR-Kinase domains aa 1329…Available SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP G116
Plasmid#123082PurposeGateway entry clone encoding human GABARAP G116DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneMutationDeleted amino acid 117. Stop codon after G116Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 MERTK full length 3'UTR
Plasmid#26992DepositorInsertMERTK Full length 3' UTR (MERTK Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-deltaY20
Plasmid#58459PurposeExpresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 20 deletedPromoterCMV IEAvailable SinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rac1(H40,L61)
Plasmid#11403DepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
TagsYFPExpressionMammalianMutationObtained with Glutamine 61 mutated to Leucine. Th…Available SinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-Y20A
Plasmid#58460PurposeExpresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-COR-Y1699C
Plasmid#25070DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains only the COR domain aa535-1838 Ty…Available SinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TYK2
Plasmid#67133PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4CTF-PY3 mutant
Plasmid#17799DepositorInsertErbB4 (ERBB4 Human)
TagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment, amino acids 676-1292, …Available SinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4CTF-PY1 mutant
Plasmid#17798DepositorInsertErbB4 (ERBB4 Human)
TagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment, amino acids 676-1292, …Available SinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf187
Plasmid#12875PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf187
ExpressionMammalianAvailable SinceDec. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(Y557C)
Plasmid#209695PurposeExpresses MAC3-tagged CCDC22(Y557C) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
TagsMAC3-tagExpressionMammalianMutationY557C, changed Tyrosine 557 to CysteineAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
Tags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRH3175
Plasmid#210177PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
ExpressionYeastMutationR33G,G141SAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-ATG13(Y118D)
Plasmid#188638PurposeFor expression of human ATG13(Y118D)DepositorInsertATG13 (ATG13 Human)
UseRetroviralTagsHAExpressionMammalianMutationChanged tyrosine 118 to aspartic acidAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-2xTS
Plasmid#162779PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M
Plasmid#140454PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, tyrosine 616 to methioni…Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:Y696G
Plasmid#98156PurposeExpresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:Y696G
TagsHis6-TEVExpressionBacterialMutationChanged tyrosine 696 to glycinePromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
TagsGST tag and His tagExpressionInsectPromoterPolyhedrinAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-Y-L
Plasmid#153546Purposethis is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucineDepositorInserthuman TCR ζ-Chain (CD247 Human)
ExpressionMammalianMutationwith 6 Y in 3 ITAMs mutated to LPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE Y165A
Plasmid#139428PurposeGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged tyrosine 165 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-RP2-C86Y,P95L
Plasmid#86074PurposeA bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.DepositorInsertRP2 (RP2 Human)
TagsGSTExpressionBacterialMutationChanged cysteine-86 and proline-95 to tyrosine an…PromotertacAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-Y20,27A
Plasmid#58465PurposeExpresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosines 20 and 27 to alaninePromoterCMV IEAvailable SinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3-Y27A
Plasmid#58464PurposeExpresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
TagsmycExpressionMammalianMutationTyrosine 27 to alaninePromoterCMV IEAvailable SinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pTH-iCre:EGFP-WPREpA
Plasmid#213142PurposeTruncated rat TH promoter expressing iCre fused to EGFPDepositorInsertiCre
UseAAVTagsEGFPExpressionMammalianPromoterTruncated TH promoter from rat (Rattus norvegicus…Available SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pTH-iCre-WPREpA
Plasmid#213130PurposeTruncated rat TH promoter expressing iCreDepositorInsertiCre
UseAAV and Mouse TargetingTagsNoneExpressionMammalianPromoterTruncated TH promoter from rat (Rattus norvegicus…Available SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)DsRed
Plasmid#200114PurposeFluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
ExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence, no other pr…Available SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-puro-PTPIP51(1-470)
Plasmid#170536PurposeExpresses PTPIP51(1-470) in human HeLa cells after transfectionDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsFLAG tagExpressionMammalianPromoterCAG promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(2xD-E_inD)Luc
Plasmid#200109PurposeLuciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertmodified EGR1 promoter
ExpressionMammalianMutationhighly modified sequencePromoter2 copies of D-E element in front of EGR1 promoter…Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapErk)Luc
Plasmid#200112PurposeLuciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activityDepositorInsertMapErk sequence in minimal promoter
ExpressionMammalianMutationhighly modified sequencePromoter5 copies of designed MapErk sequence in minimal p…Available SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
STAT3(Y705F)-TAL-Luc
Plasmid#46933PurposeLuciferase Reporter for mutant STAT3DepositorInsertSTAT3(Y705F) (STAT3 Human)
UseLuciferaseTagsFLAGMutationTyrosine 705 to PhenylalaninePromoterTA LuciferaseAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
peGFP C3-SPG20-PAAA
Plasmid#218581PurposeExpresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCHDepositorInsertSPG20 (SPART Human)
TagseGFPExpressionMammalianMutationchanged Proline 172 to Alanine and Tyrosine 174 t…PromoterCMVAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only