We narrowed to 11,713 results for: NSI

1. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

   Plasmid

   #172985

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBItetDT480GFP

   Plasmid

   #96905

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Expression

   Mammalian

   Promoter

   tetracycline responsive and bidirectional

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100852

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-FLAG-MTOR-L1460P

   Plasmid

   #69006

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-L1460P (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Leucine 1460 to Proline

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-2XFL-wtSrc

   Plasmid

   #140295

   Purpose

   Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src wildtype (SRC Human)
   

   Tags

   2X Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. peGFP C3-SPG20-PAAA

   Plasmid

   #218581

   Purpose

   Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Proline 172 to Alanine and Tyrosine 174 t…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST40-2XHA-Src-K298M

   Plasmid

   #140314

   Purpose

   Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-HNRNPA1C-mCh-Cry2WT

   Plasmid

   #101226

   Purpose

   hnRNPA1(186-320) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   hnRNPA1(186-320) (HNRNPA1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGPTVII-nlsSED1

   Plasmid

   #178190

   Purpose

   SED1 biosensor for expression in Arabidopsis thaliana

   Depositor

   Cesar Cuevas-Velazquez, Jose Dinneny

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   pUBQ10

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-HOT-mNeon-P2A-T2A-mCherry-CAAX

    Plasmid

    #240799

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via a P2A-T2A tandem

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen-mCherry-CAAX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX

    Plasmid

    #240800

    Purpose

    CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter

    Depositor

    Hans Clevers

    Article

    Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

    Insert

    mNeonGreen mCherry-CAAX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. EFS-SpdCas9

    Plasmid

    #210703

    Purpose

    This plasmid express EFS promoter driven human codon optimized SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a core promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-pmExRai-AKAR2

    Plasmid

    #161755

    Purpose

    Plasma membrane-targeted ExRai-AKAR2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    Lyn-ExRai-AKAR2
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-sRGECO

    Plasmid

    #137125

    Purpose

    Viral expression of sRGECO

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    sRGECO
    

    Use

    AAV

    Mutation

    E217D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEBG‐AMPK
α1(1‐312)

    Plasmid

    #27632

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    AMPK
α1(1‐312) (Prkaa1 Rat)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Constitutively
 active
 by 
truncation 
after 
am…

    Available Since

    May 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. NSK021 CMV-TO-p53R248Q (FLP-IN)

    Plasmid

    #191167

    Purpose

    Expression of human p53 mutant R248Q in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    p53(R248Q) (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R248Q

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-E-cadherin promoter

    Plasmid

    #61798

    Purpose

    Luciferase reporter containing the mouse E-cadherin promoter

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcdna3 flag - ULK1 wt

    Plasmid

    #27636

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse ULK1 (Ulk1 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed.

    Available Since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-anti-mCherry(LaM8) PAGER(TF)

    Plasmid

    #229999

    Purpose

    Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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