We narrowed to 7,042 results for: SIM

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pBabe puro RalA S31N

Plasmid

#19718

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

S31N mutant for dominant neg function. NOTE: lo…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

Plasmid

#60661

Purpose

Flp-dependent expression of Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

FLEX- Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Use

AAV

Promoter

EF1α

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

Plasmid

#246438

Purpose

All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

NmeCas9
Nme-sgRNA

Tags

HA tag, NLS, and SV40 NLS

Expression

Mammalian

Mutation

changed Aspartic acid 16 to Alanine, deleted amin…

Promoter

EF-1 alpha and U6

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT sfGPF 150TAG

Plasmid

#154766

Purpose

plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, U25C in PylT

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS

Plasmid

#140009

Purpose

Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mDIS3-FLAG

Plasmid

#60046

Purpose

Mammalian expression of FLAG-tagged mouse DIS3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

DIS3 (Dis3 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pDIO-DSE-mCherry-PSE-MCS

Plasmid

#129669

Purpose

Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

Depositor

Beatriz Rico

Article

Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

Type

Empty backbone

Use

AAV

Tags

mCherry

Promoter

EF1a

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-E3

Plasmid

#242408

Purpose

Self-amplifying RNA (saRNA) E3 construct; expresses the vaccinia virus E3 protein, which binds and sequesters dsRNA broadly inhibiting dsRNA-sensing pathways

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-E3

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mx1201 PylRS

Plasmid

#154764

Purpose

plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-G1 PylRS

Plasmid

#154763

Purpose

plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

Plasmid

#187356

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-1

Plasmid

#72680

Purpose

Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

lower strength RBS before mutL E32K allele than i…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pOTTC840 - pAAV 5xATF6 secNLuc

Plasmid

#82497

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

5x(ATF6 binding site) c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Article

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Tags

GFP derived from pMDC107

Expression

Plant

Promoter

35s core promoter

Available Since

Nov. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pRExT2A-NT-PmNG

Plasmid

#232200

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mNeongreen

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

Plasmid

#191164

Purpose

Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP (additional cutsites)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBA-mKeima-PIM

Plasmid

#174650

Purpose

Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

xXFKBP homo-mKeima-2x FKBP homo-2x FKBP

Expression

Mammalian

Mutation

Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

Promoter

Chicken beta-actin

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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