165,652 results

1. pWXC077

   Plasmid

   #222960

   Purpose

   C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet3
   

   Expression

   Worm

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JMJD1BA-c068

   Plasmid

   #53627

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   JMJD1BA
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLenti6MFV SLC25A4

   Plasmid

   #194133

   Purpose

   Expresses ANT1 (SLC25A4) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC25A4 (SLC25A4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJ211_rha_WT_low

   Plasmid

   #128851

   Purpose

   Low copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Expression

   Bacterial

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2/EV

   Plasmid

   #120833

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST17-UbE2R2

   Plasmid

   #15806

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. FKBP-V5-AP-nes_pLX304

   Plasmid

   #120912

   Purpose

   Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

   Depositor

   Alice Ting

   Article

   Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

   Insert

   FKBP-V5-AP-NES
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Promoter

   EF-1 alpha

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

   Plasmid

   #158990

   Purpose

   Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP64
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PS-CFP2-MyosinIIA-C-18

    Plasmid

    #57241

    Purpose

    Localization: Cytoskeleton, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    MyosinIIA (Myh9 Mouse)
    

    Tags

    PS-CFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEGFP-sfCherry11x4-ß-actin

    Plasmid

    #70220

    Purpose

    Expresses sfCherry11x4-ß-actin in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    sfCherry11x4-actin (ACTB Human)
    

    Tags

    sfCherry11x4

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA flag PPAR gamma

    Plasmid

    #8895

    Purpose

    Mammalian expression of flag-tagged PPAR gamma

    Depositor

    Bruce Spiegelman

    Article

    Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

    Insert

    PPAR gamma (Pparg Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-Fibrillarin

    Plasmid

    #26673

    Depositor

    Sui Huang

    Article

    Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-Sestrin2 (E451Q)

    Plasmid

    #237420

    Purpose

    Transient expression of N-terminally HA-tagged human Sestrin2 (E451Q)

    Depositor

    David Sabatini

    Article

    Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

    Insert

    Sestrin2 (SESN2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E451Q

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

    Plasmid

    #238181

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVeBtA2

    Plasmid

    #238987

    Purpose

    Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3VEA3

    Plasmid

    #234796

    Purpose

    Mammalian expression of bovine arrestin-3

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid

    #231552

    Purpose

    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    F-tractin (Itpka Rat)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid

    #231556

    Purpose

    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Lifeact (ABP140 Budding Yeast)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKanB

    Plasmid

    #223523

    Purpose

    Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

    Depositor

    Geoff Lin-Cereghino

    Article

    Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    Sept. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. P1K-3HA-Atg8∆R-MET15

    Plasmid

    #207034

    Purpose

    Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆R

    Promoter

    pATG8

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pROF021

    Plasmid

    #155326

    Purpose

    Expression of PULSE-controlled FLuc

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    (etr)8-(C120)5-PhCMVmin-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. TFORF2476

    Plasmid

    #144030

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZFHX2 (ZFHX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pD649-HAsp-CD70-COMP5AP-AviTag-9xHis

    Plasmid

    #157113

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CD70 (CD70 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pFRTpsiCHECK_SEAP

    Plasmid

    #154461

    Purpose

    Expression of SEAP and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    SEAP
    

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. H2B-HT-emiRFP703

    Plasmid

    #183985

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    H2B (H2BC11 Human)
    

    Tags

    HaloTag, emiRFP703

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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