161,273 results

1. pRSFDuet-1_VNp-mNeongreen-StefinA

   Plasmid

   #182419

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-stefin (CSTA Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV_BiSSTe4_GCaMP6f

   Plasmid

   #213947

   Purpose

   AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Galphai3-mTurquoise2

   Plasmid

   #69622

   Purpose

   The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Insert

   Galphai3-mTurquoise2
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sAB-K29

   Plasmid

   #204735

   Purpose

   synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

   Depositor

   Minglei Zhao

   Article

   Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

   Insert

   synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNCS mRFP

   Plasmid

   #91765

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

   Insert

   monomeric Red Fluorescent Protein1
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MCP-M-MLV RT∆RNase H

   Plasmid

   #213749

   Purpose

   For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hHS1-M7A

   Plasmid

   #159170

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1-M7A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Met 7 of the cyt b562 module of hHS1 to A…

   Promoter

   CMV

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBPGAL4.2::VP16Uw

   Plasmid

   #26228

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   GAL4::VP16 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDisplay-mSA-EGFP-TM

    Plasmid

    #39863

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin, EGFP, TM helix
    

    Use

    Tags

    EGFP, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_BRCA1_WT

    Plasmid

    #82196

    Purpose

    Gateway Donor vector containing BRCA1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLENTI_hACE2_PURO

    Plasmid

    #155295

    Purpose

    Lentiviral vector to generate hACE2 stable expressing cell line

    Depositor

    Raffaele De Francesco

    Insert

    hACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    C9

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pPB.DEST-ITGB1(WT)-mRuby2

    Plasmid

    #215448

    Purpose

    Piggybac expression vector with integrin beta1 tagged with mRuby2

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Transposon-based stable expression

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Silent mutations added to disrupt shRNA binding a…

    Promoter

    CAG

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pISH-Pax6

    Plasmid

    #74350

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax6. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    paired box 6 (Pax6 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCW-Flag-CtIP-T847E

    Plasmid

    #71110

    Purpose

    mammalian expession of Flag-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S SARS-COV-2, Synthetic, Human)
    SARS-Cov-2 E protein (E SARS-COV-2, Synthetic, Human)
    SARS-Cov-2 M Protein (M SARS-COV-2, Synthetic, Human)
    

    Use

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. 6xHis-MBP-huTsCas12a

    Plasmid

    #174687

    Purpose

    Expresses 6xHis-MBP tagged TsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huTsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMP71-BFP

    Plasmid

    #141355

    Purpose

    pMP71 expression vector containing BFP

    Depositor

    Ton Schumacher

    Article

    Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

    Insert

    tagBFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-PRMT3(E338Q)

    Plasmid

    #164696

    Purpose

    Expression of PRMT3(E338Q) with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

    Insert

    PRMT3 (PRMT3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E338Q

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. msfGFP-Cx43

    Plasmid

    #69024

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDisplay-NE2h-IRES-mCherry-CAAX

    Plasmid

    #208691

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-CAG-flex-MCS--4x6T

    Plasmid

    #196420

    Purpose

    Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZE21/UBP1/ClpS_V65I

    Plasmid

    #98567

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains the V65I mutation that increases discrim…

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hSyn-BiPOLES-mCerulean

    Plasmid

    #154944

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLL5.0-MRLC-DD-GFP

    Plasmid

    #187260

    Purpose

    Lentiviral expression of myosin regulatory light chain-DD mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18D, S19D

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLL5.0-MRLC-AA-GFP

    Plasmid

    #187259

    Purpose

    Lentiviral expression of myosin regulatory light chain-AA mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18A,S19A

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. COVID-SARS2 NSP13

    Plasmid

    #159614

    Purpose

    Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

    Depositor

    Opher Gileadi

    Article

    Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

    Insert

    Covid-SARS2 Nsp13
    

    Use

    Tags

    His6-Zb-TEV

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    T7 - LacO

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC1-HyPer-Red-C199S

    Plasmid

    #48252

    Purpose

    Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED-C199S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p-dCas9-HDAC1-Hygro

    Plasmid

    #104409

    Purpose

    transient expression of dCas9-HDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge