178,767 results
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Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mVenus NB
Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LAMP-mCherry-CRY2
Plasmid#102249PurposeExpresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
TagsHisExpressionBacterialAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
px459 VQR
Plasmid#101715PurposesgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 VQR
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVQR (D1135V, R1335Q and T1337R)Available sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4-AARE-luc2P-Hygro
Plasmid#101787PurposeLuferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xAARE
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZac2.1 GfaABC1D hM3D mCherry SV40
Plasmid#92284PurposeAAV DREADD expressionDepositorAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBluescript-CTG-47
Plasmid#99150Purpose47xCAG repeats in pBluescript vectorDepositorInsert47xCTG
ExpressionBacterialPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV_sfCherry2(11)_TagBFP
Plasmid#82606PurposeExpresses sfCherry2(11) and TagBFP fusion in mammalian cellsDepositorInsertsfCherry2(11)_TagBFP
UseLentiviralExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterSFFVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Tau
Plasmid#92201PurposeRetroviral overexpression vector (doxycycline-inducible) for TauDepositorAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-ATP6V0D1
Plasmid#87931PurposeFLAG-tagged overexpressionDepositorAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ220 (AsCpf1)
Plasmid#86208PurposeAsCpf1 Gateway entry plasmidDepositorInsertAsCpf1
UseGateway compatible cpf1 vectorsTags5' and 3' NLSExpressionPlantAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-1BPNLS-Cas9-1BPNLS
Plasmid#87108PurposeBPNLS-Cas9-BPNLS expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1BPNLS-Cas9-1BPNLS
UseCRISPRTags3xFLAGExpressionMammalianPromoterCAGAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV9:cTNT::3Flag-hYAP S127A
Plasmid#86558PurposeTo produce cardiac specific adeno-associated virus expressing activated human YAP.DepositorInsertYAP (YAP1 Human)
UseAAVTags3FlagMutationSerine 127 was mutated into Alanine, to activate …PromotercTNTAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pF CAG luc puro
Plasmid#67501PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionMammalianPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsF2AExpressionMammalianPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 mCherry-Atl3
Plasmid#86680PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfluorescent protein voltage sensor
TagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAW016-2
Plasmid#85613PurposeCas9-tracrRNA integration vector for Bacillus subtilisDepositorInsertcas9-tracrRNA
ExpressionBacterialAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti Lifeact-mRuby2 BlastR
Plasmid#84384PurposeLentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2PromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML104-HygMx4
Plasmid#83475PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.DepositorInsertHphMX4
ExpressionYeastAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pscAAV-CAG-RLuc
Plasmid#83280Purposerecombinant AAV vector packaging self-complementary Renilla Luciferase under the CAG promoterDepositorInsertRenilla Luciferase
UseAAVExpressionMammalianPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPaint-TagGFP2
Plasmid#67166PurposeCRISPaint universal donor for TagGFP2 fusion taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagGFP2
Available sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_Flag_NS4A
Plasmid#79636PurposeThird generation Self-inactivating lentivector expressing NS4A from Zika virusDepositorInsertNS4A
UseLentiviralTagsFlagExpressionMammalianPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUB_smHA_KDM5B_MS2
Plasmid#81085PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmHA_KDM5B_24xMS2
ExpressionMammalianAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CAG-DIO-APEX2NES
Plasmid#79907PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NESDepositorInsertAPEX2NES
UseAAV and Cre/LoxTagsV5ExpressionMammalianAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLAMP1-miRFP703
Plasmid#79998PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP703DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1 (Lamp1 Rat)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by