165,651 results

1. XX01CAPN1A-c001

   Plasmid

   #175491

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN1 / CAPNS1 (CAPN1 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-Rab7A

   Plasmid

   #28047

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-HLA-A2

   Plasmid

   #135504

   Purpose

   Mammalian expression of myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAK_DR30_CasRx_Puro

   Plasmid

   #134845

   Purpose

   All in one overexpression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   T2A Puromycin

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hKDR

   Plasmid

   #83436

   Purpose

   human VEGFR2 with V5 and tGFP tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

   Insert

   human VEGFR2 (KDR Human)
   

   Tags

   V5 and turboGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. iC13 PB-TO-oNgn2-BFP-puro

   Plasmid

   #204733

   Purpose

   Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Optimized neurogenin-2
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HiFi Cas9

   Plasmid

   #188490

   Purpose

   Expresses FLAG tagged HiFi Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   R691A

   Promoter

   EF1a

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-G1mCNPRS

   Plasmid

   #174718

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EBFP2-N1

    Plasmid

    #54595

    Purpose

    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    EBFP2

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid

    #207771

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMRE-Tn5-160

    Plasmid

    #118540

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LM670

    Plasmid

    #191552

    Purpose

    LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

    Depositor

    David Baker

    Article

    Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

    Insert

    ccdb
    

    Tags

    HIS-tag

    Expression

    Bacterial

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GW1-Mito-pHRed

    Plasmid

    #31474

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    Cox8-tagged pHRed
    

    Tags

    4xCox8

    Expression

    Mammalian

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p20StAD

    Plasmid

    #190268

    Purpose

    Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

    Depositor

    Assaf Mosquna

    Article

    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

    Type

    Empty backbone

    Use

    Synthetic Biology; Yeast genome-integrating vector

    Tags

    GAL4-AD

    Promoter

    TDH3

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCytERM-mTagBFP2

    Plasmid

    #184940

    Purpose

    ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-mTagBFP2
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX4T-hLIG3

    Plasmid

    #81055

    Purpose

    bacterial expression of human LIG3

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    DNA ligase 3 (LIG3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEBTet-SNAP-Cep72

    Plasmid

    #136819

    Purpose

    Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep72 (CEP72 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

    Plasmid

    #175522

    Purpose

    Nuclear expression of HaloTag11 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag11
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSOX11-donor

    Plasmid

    #176360

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    SOX11 (SOX11 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Available Since

    Nov. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLV-CMV-KIF2C

    Plasmid

    #205994

    Purpose

    Over-expression of GFP-KIF2c

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    KIF2c (KIF2C Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pACUH-GFP1-10 mitochondrial matrix

    Plasmid

    #172063

    Purpose

    UAS vector to express GFP1-10 in the mitochondrial matrix

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP1-10 mitochondrial matrix
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. LeGO-iG

    Plasmid

    #27358

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, IRES-eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. Beta1-GFP in pHcgreen

    Plasmid

    #69804

    Purpose

    Expresses Beta1-GFP integrin for normal cell transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1A integrin (ITGB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.1-2xFLAG-2xSTREP-CCND2

    Plasmid

    #172629

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. LV-miR-142

    Plasmid

    #117056

    Purpose

    Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

    Depositor

    Michele De Palma

    Article

    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

    Insert

    miR-142-3p - OFP (Mir142 Mouse)
    

    Use

    Lentiviral

    Promoter

    SFFV promoter

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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