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We narrowed to 82,376 results for: TRI

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Showing: 10381 - 10410 of 82376 results

  1. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  3. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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  4. psiCHECK2-let-7 WT

    Plasmid
    #78260
    Purpose
    psiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    fully complimentary let-7 target site
    Use
    Luciferase; Microrna activity
    Expression
    Mammalian
    Available Since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78260-plasmid-map-sequence-id-149453

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  13. pJAB988

    Plasmid
    #133920
    Purpose
    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter
    Depositor
    Christopher Voigt
    Article
    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.
    Insert
    miniICE-tet(M)cat(PC194)_PT7-GFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    133920-plasmid-map-sequence-id-409802

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  14. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Article
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Insert
    LOV2 and STIM1 cytosolic fragment
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101245-plasmid-map-sequence-id-209272

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  19. pIBA3-SpyCatcher-sfGFP

    Plasmid
    #107420
    Purpose
    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107420-plasmid-map-sequence-id-207599

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  24. eGFP-hCOL1A1

    Plasmid
    #140110
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag, K20…
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140110-plasmid-map-sequence-id-276323

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Showing: 10381 - 10410 of 82376 results