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pHS-393

Plasmid

#195473

Purpose

Lentiviral Integratable Inducible super-IL2 cytokine + synZiFTR10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

inverted[8X ZF10 BS - ybTATA - IL12] - pSFFV - ZF10 - NS3 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAP114

Plasmid

#120799

Purpose

E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

Insert

Pxyl::mCherryOpt

Expression

Bacterial

Mutation

1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

Promoter

Pxyl

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Bst-LF

Plasmid

#145799

Purpose

Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

Depositor

Andrew Ellington

Article

Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

Insert

Bst-LF

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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MiniCoopR 2xU6:gRNA, mitfa:Cas9

Plasmid

#118844

Purpose

Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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p8092 LentiCRISPR v2 sgNT-1

Plasmid

#163315

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgNT-1

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKEK3230

Plasmid

#234102

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

FuGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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plk1 T210A

Plasmid

#132965

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 T210A (PLK1 Human)

Expression

Mammalian

Mutation

T210A

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP8923

Plasmid

#220904

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLG027 (qatABCD)

Plasmid

#157905

Purpose

Expresses the qatABCD defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

Plasmid

#205152

Purpose

Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

FRT-GFP-FRT-uORF-dCBEevoCDA1

Use

CRISPR

Expression

Insect

Promoter

UAST

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-Myc-gate-pGK-HYG

Plasmid

#107395

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

Myc

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-cyan pre-eGRASP(p30)

Plasmid

#111586

Purpose

An AAV vector that expresses cyan pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMF-FU_TetO_GW_PPARG2

Plasmid

#193289

Purpose

Lentiviral construct that expresses a dox-inducible human PPARG2

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

PPARG2 (PPARG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_07_01

Plasmid

#165014

Purpose

Expression of HLA-DRB1*07:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*07:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK4

Plasmid

#122675

Purpose

Expresses mouse PCSK4 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)

Expression

Mammalian

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304-EV-IRES-GFP

Plasmid

#187640

Purpose

Empty Vector control modified to express GFP

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

EV

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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HLA_DRA_01_01

Plasmid

#165012

Purpose

Expression of HLA-DRA*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRA (HLA-DRA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pZR060_CROPseq-sgCtrl-CRISPRi

Plasmid

#180269

Purpose

Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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